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Evaluation of methods for single hair DNA amplification
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Evolution, Genomics and Systematics, Evolutionary Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Evolution, Genomics and Systematics, Evolutionary Biology.
2007 (English)In: Conservation Genetics, ISSN 1566-0621, E-ISSN 1572-9737, Vol. 8, no 4, 977-981 p.Article in journal (Refereed) Published
Abstract [en]

Because of the low amount of DNA in single hairs, it may be difficult to obtain reliable genotypes for forensic and conservation genetics studies. We therefore compared different methods for reliably genotyping single hair samples. Our results indicate that preliminary whole genome amplification can increase the likelihood of successfully genotyping a single hair compared to other commonly used protocols. The difference between the methods is small for single locus comparisons, but it becomes more important in multi-locus comparisons. The economic and time costs of the whole genome amplification may prevent its large-scale use in non-invasive monitoring programs. Nevertheless, it may be a very useful approach for the analysis of especially valuable samples.

Place, publisher, year, edition, pages
2007. Vol. 8, no 4, 977-981 p.
Keyword [en]
Chelex, Dropout, GenomiPhi, Noninvasive monitoring, Whole genome amplification
National Category
Biological Sciences
URN: urn:nbn:se:uu:diva-95707DOI: 10.1007/s10592-006-9220-zISI: 000248300300019OAI: oai:DiVA.org:uu-95707DiVA: diva2:170022
Available from: 2007-04-12 Created: 2007-04-12 Last updated: 2016-04-25Bibliographically approved
In thesis
1. Consequences of the Domestication of Man’s Best Friend, The Dog
Open this publication in new window or tab >>Consequences of the Domestication of Man’s Best Friend, The Dog
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The dog was the first animal to be domesticated and the process started at least 15 000 years ago. Today it is the most morphologically diverse mammal, with a huge variation in size and shape. Dogs have always been useful to humans in several ways, from being a food source, hunting companion, guard, social companion and lately also a model for scientific research.

This thesis describes some of the changes that have occurred in the dog’s genome, both during the domestication process and later through breed creation. To give a more comprehensive view, three genetic systems were studied: maternally inherited mitochondrial DNA, paternally inherited Y chromosome and biparental autosomal chromosomes. I also sequenced complete mitochondrial genomes to view the effect new living conditions might have had on dogs’ genes after domestication. Finally, knowledge of the genetic structure in purebred dogs was used to test analytic methods usable in other species or in natural populations where little information is available.

The domestication process appears to have caused a relaxation of the selective constraint in the mitochondrial genome, leading to a faster rate of accumulation of nonsynonymous changes in the mitochondrial genes. Later, the process of breed creation resulted in genetically separated breed groups. Breeds are a result from an unequal contribution of males and females with only a few popular sires contributing and a larger amount of dams. However, modern breeder preferences might lead to disruptive selective forces within breeds, which can result in additional fragmentation of breeds. The increase in linkage disequilibrium that this represents increases the value of purebred dogs as model organisms for the identification and mapping of diseases and traits. Purebred dogs’ potential for these kinds of studies will probably increase the more we know about the dog’s genome.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2007. 64 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 289
Molecular genetics, mitochondrial genome, Y chromosome, microsatellites, disruptive selection, breed structure, whole genome amplification, gene flow, linkage disequilibrium, Canis familiaris, Canis lupus, Genetik
urn:nbn:se:uu:diva-7799 (URN)978-91-554-6854-5 (ISBN)
Public defence
2007-05-03, Zootissalen, Zoologisk institution, EBC, Villavägen 9, Uppsala, 09:00 (English)
Available from: 2007-04-12 Created: 2007-04-12 Last updated: 2009-03-31Bibliographically approved

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