Self-assembly of proximity probes for flexible and modular proximity ligation assays
2007 (English)In: BioTechniques, ISSN 0736-6205, Vol. 43, no 4, 443-450 p.Article in journal (Refereed) Published
Proximity ligation assay (PLA) is a recently developed strategy for protein analysis in which antibody-based detection of a target protein via a DNA ligation reaction of oligonucleotides linked to the antibodies results in the formation of an amplifiable DNA strand suitable for analysis. Here we describe a faster and more cost-effective strategy to construct the antibody-based proximity ligation probes used in PLA that is based on the noncovalent interaction of biotinylated oligonucleotides with streptavidin followed by the interaction of this complex with biotinylated antibodies.
Place, publisher, year, edition, pages
2007. Vol. 43, no 4, 443-450 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-95759DOI: 10.2144/000112551ISI: 000205886000019PubMedID: 180119334OAI: oai:DiVA.org:uu-95759DiVA: diva2:170091