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DNA content and nucleoid distribution in Methanothermobacter thermautotrophicus
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Evolution, Genomics and Systematics, Molecular Evolution.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Evolution, Genomics and Systematics, Molecular Evolution.
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2005 (English)In: Journal of Bacteriology, ISSN 0021-9193, E-ISSN 1098-5530, Vol. 187, no 5, 1856-1858 p.Article in journal (Refereed) Published
Abstract [en]

Flow cytometry and epifluorescence microscopy results for the euryarchaeon Methanothermobacter thermautotrophicus were consistent with filaments containing multiple cells. Filaments of one to four cells contained two to eight nucleoids. Single chromosome-containing cells were not observed. Filaments containing multiple genome copies displayed synchronous DNA replication initiation. Chromosome segregation occurred during replication or rapidly after replication termination.

Place, publisher, year, edition, pages
2005. Vol. 187, no 5, 1856-1858 p.
National Category
Biological Sciences
URN: urn:nbn:se:uu:diva-95837DOI: 10.1128/JB.187.5.1856-1858.2005ISI: 000227191600035PubMedID: 15716458OAI: oai:DiVA.org:uu-95837DiVA: diva2:170191
Available from: 2007-04-26 Created: 2007-04-26 Last updated: 2011-02-18Bibliographically approved
In thesis
1. Exploring the Cell Cycle of Archaea
Open this publication in new window or tab >>Exploring the Cell Cycle of Archaea
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Archaea is the third domain of life, discovered only thirty years ago. In a microscope archaea appear indistinguishable from bacteria, but they have been shown to be more closely related to eukaryotes than to bacteria. Especially central information processing is homologous to that of eukaryotes. The archaea, previously thought to be limited to extreme environments, constitute a large part of life on Earth to an extent that has only begun to be understood. Despite their abundance little is known about several central cell-cycle features, such as cell division and genome segregation.

For this thesis, a comprehensive study of the cell cycle in the model archaeon Sulfolobus acidocaldarius was performed, describing the majority of its cell-cycle regulated genes. Several known DNA replication genes, as well as genes previously not known to have a role in the cell cycle, displayed cyclic transcription. Several transcription factors, kinases and DNA sequence elements were identified as cell-cycle regulatory elements. Among the most important findings were putative cell division and genome segregation machineries.

Sulfolobus species were discovered to have three origins of replication, constituting the first known prokaryotes with multiple origins. All origins initiate replication in a synchronous manner. Cdc6 proteins were shown to bind to origin recognition boxes conserved across the Archaea domain. Two Cdc6 proteins function as replication initiators, while a third paralog is implicated as a negative factor. Replication was shown to proceed at a rate similar to that of eukaryotes.

A particular type of cell cycle organization was found to be unusually conserved in the Crenachaeota phylum. All the studied species displayed a short prereplicative phase and a long postreplicative phase, and cycle between one and two genome copies. Genome sizes were determined for several species. The euryarchaeon Methanothermobacter thermautotrophicus was also studied, and it was shown to initiate genome segregation during, or just after, replication. In contrast to the crenarchaea it never displayed a single genome copy per cell.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2007. 71 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 300
Microbiology, Archaea, Cell cycle, Replication, Mitosis, Cell division, Mikrobiologi
urn:nbn:se:uu:diva-7848 (URN)978-91-554-6881-1 (ISBN)
Public defence
2007-05-18, Lindahlsalen, Evolutionsbiologiskt centrum, Norbyvägen 18, Uppsala, 13:15
Available from: 2007-04-26 Created: 2007-04-26Bibliographically approved

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