Genome-wide transcription map of an archaeal cell cycle
2007 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 104, no 8, 2939-2944 p.Article in journal (Refereed) Published
Relative RNA abundance was measured at different cell-cyclestages in synchronized cultures of the hyperthermophilic archaeonSulfolobus acidocaldarius. Cyclic induction was observed for >160genes, demonstrating central roles for transcriptional regulationand cell-cycle-specific gene expression in archaeal cell-cycle pro-gression. Many replication genes were induced in a cell-cycle-specific manner, and novel replisome components are likely to beamong the genes of unknown function with similar inductionpatterns. Candidate genes for the unknown genome segregationand cell division machineries were also identified, as well as seventranscription factors likely to be involved in cell-cycle control. Twoserine-threonine protein kinases showed distinct cell-cycle-specificinduction, suggesting regulation of the archaeal cell cycle alsothrough protein modification. Two candidate recognition ele-ments, CCR boxes, for transcription factors in control of cell-cycleregulons were identified among gene sets with similar inductionkinetics. The results allow detailed characterization of the genomesegregation, division, and replication processes and may, becauseof the extensive homologies between the archaeal and eukaryoticinformation machineries, also be applicable to core features of theeukaryotic cell cycle.
Place, publisher, year, edition, pages
2007. Vol. 104, no 8, 2939-2944 p.
Archaea, chromosome replication, microarrays, mitosis, Sulfolobus
IdentifiersURN: urn:nbn:se:uu:diva-95838DOI: 10.1073/pnas.0611333104ISI: 000244511200067PubMedID: 17307872OAI: oai:DiVA.org:uu-95838DiVA: diva2:170192