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Genome-wide transcription map of an archaeal cell cycle
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Evolution, Genomics and Systematics, Molecular Evolution.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Evolution, Genomics and Systematics, Molecular Evolution.
2007 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 104, no 8, 2939-2944 p.Article in journal (Refereed) Published
Abstract [en]

Relative RNA abundance was measured at different cell-cyclestages in synchronized cultures of the hyperthermophilic archaeonSulfolobus acidocaldarius. Cyclic induction was observed for >160genes, demonstrating central roles for transcriptional regulationand cell-cycle-specific gene expression in archaeal cell-cycle pro-gression. Many replication genes were induced in a cell-cycle-specific manner, and novel replisome components are likely to beamong the genes of unknown function with similar inductionpatterns. Candidate genes for the unknown genome segregationand cell division machineries were also identified, as well as seventranscription factors likely to be involved in cell-cycle control. Twoserine-threonine protein kinases showed distinct cell-cycle-specificinduction, suggesting regulation of the archaeal cell cycle alsothrough protein modification. Two candidate recognition ele-ments, CCR boxes, for transcription factors in control of cell-cycleregulons were identified among gene sets with similar inductionkinetics. The results allow detailed characterization of the genomesegregation, division, and replication processes and may, becauseof the extensive homologies between the archaeal and eukaryoticinformation machineries, also be applicable to core features of theeukaryotic cell cycle.

Place, publisher, year, edition, pages
2007. Vol. 104, no 8, 2939-2944 p.
Keyword [en]
Archaea, chromosome replication, microarrays, mitosis, Sulfolobus
National Category
Biological Sciences
URN: urn:nbn:se:uu:diva-95838DOI: 10.1073/pnas.0611333104ISI: 000244511200067PubMedID: 17307872OAI: oai:DiVA.org:uu-95838DiVA: diva2:170192
Available from: 2007-04-26 Created: 2007-04-26 Last updated: 2011-02-11Bibliographically approved
In thesis
1. Exploring the Cell Cycle of Archaea
Open this publication in new window or tab >>Exploring the Cell Cycle of Archaea
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Archaea is the third domain of life, discovered only thirty years ago. In a microscope archaea appear indistinguishable from bacteria, but they have been shown to be more closely related to eukaryotes than to bacteria. Especially central information processing is homologous to that of eukaryotes. The archaea, previously thought to be limited to extreme environments, constitute a large part of life on Earth to an extent that has only begun to be understood. Despite their abundance little is known about several central cell-cycle features, such as cell division and genome segregation.

For this thesis, a comprehensive study of the cell cycle in the model archaeon Sulfolobus acidocaldarius was performed, describing the majority of its cell-cycle regulated genes. Several known DNA replication genes, as well as genes previously not known to have a role in the cell cycle, displayed cyclic transcription. Several transcription factors, kinases and DNA sequence elements were identified as cell-cycle regulatory elements. Among the most important findings were putative cell division and genome segregation machineries.

Sulfolobus species were discovered to have three origins of replication, constituting the first known prokaryotes with multiple origins. All origins initiate replication in a synchronous manner. Cdc6 proteins were shown to bind to origin recognition boxes conserved across the Archaea domain. Two Cdc6 proteins function as replication initiators, while a third paralog is implicated as a negative factor. Replication was shown to proceed at a rate similar to that of eukaryotes.

A particular type of cell cycle organization was found to be unusually conserved in the Crenachaeota phylum. All the studied species displayed a short prereplicative phase and a long postreplicative phase, and cycle between one and two genome copies. Genome sizes were determined for several species. The euryarchaeon Methanothermobacter thermautotrophicus was also studied, and it was shown to initiate genome segregation during, or just after, replication. In contrast to the crenarchaea it never displayed a single genome copy per cell.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2007. 71 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 300
Microbiology, Archaea, Cell cycle, Replication, Mitosis, Cell division, Mikrobiologi
urn:nbn:se:uu:diva-7848 (URN)978-91-554-6881-1 (ISBN)
Public defence
2007-05-18, Lindahlsalen, Evolutionsbiologiskt centrum, Norbyvägen 18, Uppsala, 13:15
Available from: 2007-04-26 Created: 2007-04-26Bibliographically approved

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