uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Ancient human DNA: authentication through FLX-sequenced PCR products in conjunction with bleach pre-treatment, quantitative PCR, assesment of assymetric behaviour and negative controls
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Evolution, Genomics and Systematics, Evolutionary Biology.
Show others and affiliations
(English)Manuscript (Other (popular science, discussion, etc.))
URN: urn:nbn:se:uu:diva-96067OAI: oai:DiVA.org:uu-96067DiVA: diva2:170524
Available from: 2007-08-31 Created: 2007-08-31 Last updated: 2010-01-14Bibliographically approved
In thesis
1. Ancient DNA as a Means to Investigate the European Neolithic
Open this publication in new window or tab >>Ancient DNA as a Means to Investigate the European Neolithic
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The transition from a hunter-gatherer lifestyle to a farming lifestyle, i.e. the Neolithisation, is arguably the most important event in human prehistory. While the geography and dating of the Neolithisation is well known, the process is still under debate, especially if it occurred through diffusion of ideas or with migrating farmers. The process accelerated when alternative use of domesticated animals increased. Especially the use of dairy products, and the consumption of unprocessed milk, appears to be of importance. As milk consumption (lactose digestion) is dependent upon genetic components, it is debated whether the genetic disposition allowed for dairy production to evolve, or if the usages of dairy products added selection pressure that eventually lead to present day allele frequencies. Molecular genetics have the potential to solve this and similar questions, but only if the contamination problem, where authentic DNA can be distinguished from modern contaminating DNA, can be resolved.

Here I investigate the nature and extent of contamination with modern human DNA in museum specimens and explore several approaches to minimise this contamination and to authenticate DNA results from ancient humans. I use real-time quantification, pyrosequencing and FLX-generated clonal sequencing assays to generate data on ancient humans and ancient dogs. I further use the techniques to study the development of lactase persistence and the nature of animal domestication.

The results presented show that sample-based contamination is extensive, but can be minimised if treated with bleach. I retrieved authentic HVSI sequences from 30 Neolithic hunter-gatherers and farmers from Sweden, of which eighteen also yielded nuclear data indicating that the farmers had a higher frequency of the allele linked to lactase persistence compared to the hunter-gatherers. I conclude that genetic data from ancient humans as well as from ancient animals can be retrieved and used, but only under high stringency.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2007. 70 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 323
Molecular genetics, aDNA, contamination, authentication, mtDNA, Neolithisation, Genetik
urn:nbn:se:uu:diva-8162 (URN)978-91-554-6940-5 (ISBN)
Public defence
2007-09-21, Lindhalsalen, Norbyvägen 14, Uppsala, 13:00 (English)
Available from: 2007-08-31 Created: 2007-08-31 Last updated: 2009-03-31Bibliographically approved

Open Access in DiVA

No full text

By organisation
Evolutionary Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 246 hits
ReferencesLink to record
Permanent link

Direct link