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Screening and identification of aluminium-containing biomolecules by column-switched LC-ICP-MS and LC-ESI-MS/MS
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry.
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2007 (English)In: Journal of Analytical Atomic Spectrometry, ISSN 0267-9477, E-ISSN 1364-5544, Vol. 22, no 11, 1397-1402 p.Article in journal (Refereed) Published
Abstract [en]

Column-switching liquid chromatography followed by low resolution ICP-MS was evaluated as a tool for speciation analysis of aluminium-containing biomolecules. The strategy was applied on siderophores, small organic molecules (Mr < 1500) which normally act as strong iron chelators. The drawbacks normally encounterd with aluminium detection using low resolution ICP-MS are the formation of polyatomic ions causing isobaric overlaps and space-charge effects. When adding a carbon rich solvent, such as methanol or acetonitrile, the 13C14N+, 12C15N+ and 12C14N1H+ with the same mass as 27Al+ will form in the plasma. The nitrogen is either entrained from the surrounding atmosphere or added with the constituents in the mobile phase. These disadvantages were successfully counteracted by the use of nitrogen free organic modifier in the mobile phase and the use of cool plasma conditions. Detection limits for standard solutions of aluminium-chelated ferrichrome in sub-nanomolar range were obtained by monitoring the aluminium-27 isotope. The combined use of LC-ICP-MS and LC-ESI-MS/MS was also evaluated as a tool to identify unknown metal complexes, here siderophores, in field soil solution samples. Two aluminium-chelated siderophores, Al-desferrichrom and Al-desferricrocin, were identified and quantified. Both aluminium-siderophore complexes were present in the low nanomolar range (1.1 and 0.7 nM, respectively).

Place, publisher, year, edition, pages
2007. Vol. 22, no 11, 1397-1402 p.
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-96098DOI: 10.1039/b707948fISI: 000250399600015OAI: oai:DiVA.org:uu-96098DiVA: diva2:170561
Available from: 2007-09-04 Created: 2007-09-04 Last updated: 2011-06-21Bibliographically approved
In thesis
1. Inductively Coupled Plasma Spectrometry for Speciation Analysis: Development and Applications
Open this publication in new window or tab >>Inductively Coupled Plasma Spectrometry for Speciation Analysis: Development and Applications
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In analytical chemistry the main goal is normally to determine the identity and/or concentration of one or more species in a sample. The samples analyzed are often natural samples, containing numerous different species in a complex matrix and the choice of technique for multi-elemental detection is in general inductively coupled plasma spectrometry. The chemical forms of an element can affect many of its characteristics e.g. toxicity, which makes speciation analysis important. Therefore, determination of the identity and quantity of an element is still important, but for many applications measurements of total element concentration provides insufficient information. To be able to perform speciation analysis, separation, identification and/or characterization of the various forms of elements in the sample has to be accomplished. Speciation analysis has been employed in a wide range of disciplines, including for example environmental science, biology and clinical chemistry.

This thesis describes work to improve and understand the elemental speciation analysis with liquid chromatography coupled to plasma spectrometry and also highlights the importance and potential of the synergy between atomic spectrometry and molecular mass spectrometry. The combination of the matrix tolerant, robust and very sensitive plasma spectrometry used together with molecular mass spectrometry, which provides structural information and the possibility to identify unknown species, is demonstrated to be a very powerful tool for speciation analysis. In this thesis methods are developed for on-line sample clean-up and pre-concentration coupled to liquid chromatography and plasma spectrometry, which makes handling of small sample volumes easier and also decreases the risk of contamination. The problems associated with organic modifiers in plasma spectrometry are also addressed. Applications of speciation analysis are exemplified by analysis of aluminium-chelated siderophores in field-soil solutions and organic phosphorous species in aquatic sediments. The possibility to analyze un-dissolved samples as slurries with minimal sample preparation is also discussed.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2007. 56 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 327
Keyword
Analytical chemistry, Speciation analysis, Atomic emission spectrometry (AES), Mass spectrometry (MS), Inductively coupled plasma (ICP), Liquid Chromatography (LC), on-line sample pre-treatment, Organic phosphour, Siderophores, Slurry nebulization, matrix effects, Analytisk kemi
Identifiers
urn:nbn:se:uu:diva-8169 (URN)978-91-554-6945-0 (ISBN)
Public defence
2007-09-25, B41, BMC, Uppsala, 13:15 (English)
Opponent
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Available from: 2007-09-04 Created: 2007-09-04 Last updated: 2011-05-10Bibliographically approved

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Forsgard, NiklasSjöberg, PerAndersson, MaritPettersson, Jean

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