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Antimicrobial and cytotoxic activity of agelasine and agelasimine analogs
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Division of Pharmacognosy.
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2007 (English)In: Bioorganic & Medicinal Chemistry, ISSN 0968-0896, E-ISSN 1464-3391, Vol. 15, no 12, 4016-4037 p.Article in journal (Refereed) Published
Abstract [en]

Agelasine and agelasimine derivatives with substantially less complicated terpenoid side chains compared to the naturally occurring compounds have been synthesized and their ability to inhibit growth of microorganisms and cancer cells has been studied. Compounds with excellent activity against cancer cell lines (MIC ca. 1 μM for the most potent compounds), including a drug resistant renal cell line, have been identified. Most compounds studied also exhibited broad spectrum antimicrobial activity including activity against Mycobacterium tuberculosis.

Place, publisher, year, edition, pages
2007. Vol. 15, no 12, 4016-4037 p.
Keyword [en]
Antimicrobial agent, Anti-cancer agent, Adenine derivative, Natural product
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-96232DOI: 10.1016/j.bmc.2007.03.086ISI: 000246870400007PubMedID: 17442577OAI: oai:DiVA.org:uu-96232DiVA: diva2:170735
Available from: 2007-09-27 Created: 2007-09-27 Last updated: 2011-01-28Bibliographically approved
In thesis
1. Bioactive Compounds in the Chemical Defence of Marine Sponges: Structure-Activity Relationships and Pharmacological Targets
Open this publication in new window or tab >>Bioactive Compounds in the Chemical Defence of Marine Sponges: Structure-Activity Relationships and Pharmacological Targets
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Marine invertebrates, in particular sponges, represent a source of a wide range of secondary metabolites, many of which have been attributed various defensive capabilities against environmental stress factors. In this thesis sponge-derived low-molecular peptide-like compounds and associated analogs are investigated for bioactivity and pharmacological targets.

The compound bromobenzisoxazolone barettin (cyclo[(6-bromo-8-(6-bromo-benzioxazol -3(1H)-one)-8-hydroxy)tryptophan)]arginine) was isolated from the sponge Geodia barretti and its ability to inhibit larval settlement of the barnacle Balanus improvisus was determined. With an EC50 value of 15 nM, this compound’s antifouling effect was higher than those of the previously reported brominated dipeptides from Geodia barretti, i.e., barettin and 8,9-dihydrobarettin; moreover, this antifouling effect was demonstrated to be reversible. However, the compound lacked affinity for 5-HT1-7 receptors, whereas barettin possessed specific affinity to 5-HT2A, 5-HT2C and 5-HT4, while 8,9-dihydrobarettin interacted with 5-HT4. In an attempt to evaluate structure-activity relationships synthesized analogs with barettin and dipodazine scaffolds were investigated for antifouling activity. The analog benso[g]dipodazine, with an EC50 value of 34 nM, displayed the highest settlement inhibition.

The studies of the structure-activity relationships of sponge-derived compounds were extended to cover analogs of agelasines and agelasimines originally isolated from sponges of the genus Agelas. Synthesized (+)-agelasine D and two structurally close analogs were investigated for cytotoxic and antibacterial activity. The profound cytotoxicity and broad spectrum antibacterial activity found prompted a further investigation of structure-activity relationships in 42 agelasine and agelasimine analogs and several characteristics that increased bioactivity were identified.

In conclusion this work has produced new results regarding the potent bioactivity of compounds derived from the sponges Geodia barretti and Agelas spp. and increased SAR knowledge of the fouling inhibition, cytotoxicity and antimicrobial activity of these compounds.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2007. 54 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Pharmacy, ISSN 1651-6192 ; 63
Pharmacognosy, 5-hydroxytryptamine, agelasine, agelasimine, antibacterial, antifouling, barettin, bromobenzisoxazolone barettin, cytotoxic, marine, secondary metabolite, sponge, Farmakognosi, Agelas, Geodia barretti
urn:nbn:se:uu:diva-8218 (URN)978-91-554-6971-9 (ISBN)
Public defence
2007-10-19, B7:113a, BMC, BMC, Uppsala, 13:15
Available from: 2007-09-27 Created: 2007-09-27 Last updated: 2011-06-16Bibliographically approved

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Larsson, RolfBohlin, Lars
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