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Impact of humic substances on EROD activity in gill and liver of three-spined sticklebacks (Gasterosteus aculeatus)
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Environmental Toxicology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Environmental Toxicology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Environmental Toxicology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Environmental Toxicology.
2010 (English)In: Chemosphere, ISSN 0045-6535, E-ISSN 1879-1298, Vol. 81, no 2, 156-160 p.Article in journal (Refereed) Published
Abstract [en]

Humic substances (HS) are ubiquitous in the environment and have been found to influence physiological functions of aquatic organisms. In the present study, three-spined sticklebacks (Gasterosteus aculeatus) were exposed to HS of different origins to evaluate effects on the  7-ethoxyresorufin O-deethylase (EROD) activity catalyzed by cytochrome   P4501A (CYP1A) in the liver and the gill. To that end, three-spined   sticklebacks were exposed for 48 h to different concentrations of synthetic humic acid (AHA), Nordic reservoir natural organic matter  (N.R.-NOM) and water from six lakes with different concentrations of   HS. EROD activity was significantly induced (3-6-fold) in the gills of   fish exposed to water from all lakes except the lake with the lowest   concentration of HS. All tested concentrations of AHA and N.R.-NOM   significantly induced gill EROD activity and the induction was   dose-dependent. AHA, but neither N.R.-NOM nor lake water, induced EROD activity in the liver. In addition, fish were exposed to the potent  CYP1A inducers benzo(a)pyrene (BaP) and PCB126 in combination with AHA.   Presence of AHA had no significant effect on EROD induction by BaP or   PCB126. The components in HS responsible for EROD induction remain to be identified. Our finding that HS of both natural and synthetic origin induce EROD activity in the gill is of significance for the   interpretation of biomonitoring data on EROD activity as well as for the choice of suitable reference waters.

Place, publisher, year, edition, pages
2010. Vol. 81, no 2, 156-160 p.
Keyword [en]
Humic substances, Three-spined stickleback, EROD activity, Natural organic matter
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-96246DOI: 10.1016/j.chemosphere.2010.06.073ISI: 000282155400003PubMedID: 20797764OAI: oai:DiVA.org:uu-96246DiVA: diva2:170754
Available from: 2007-09-28 Created: 2007-09-28 Last updated: 2017-12-14Bibliographically approved
In thesis
1. Evaluation of Biomarker Responses in Fish: with Special Emphasis on Gill EROD Activity
Open this publication in new window or tab >>Evaluation of Biomarker Responses in Fish: with Special Emphasis on Gill EROD Activity
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Many chemicals present in the aquatic environment can interfere with physiological functions in fish. Exposure to chemicals can be revealed by the use of biomarkers. Induction of 7-ethoxyresorufin O-deethylase (EROD) activity is a commonly used biomarker for exposure to CYP1A inducers such as dioxins and polyaromatic hyrdrocarbons. Vitellogenin is a frequently used biomarker for estrogenic compounds in various fish species whereas a biomarker for androgens, spiggin, is only found in sticklebacks. The main objectives of this thesis were to evaluate gill EROD activity as a biomarker and the three-spined stickleback as a model species in ecotoxicological studies.

EROD activities were measured in gill, liver and kidney in rainbow trout (Oncorhynchus mykiss) caged in urban areas in Sweden. EROD induction was most pronounced in the gill. Also in fish caged at reference sites, with an expected low level of known CYP1A inducers, a marked gill EROD induction was found. One suggested inducer in rural waters is humic substances (HS). To evaluate the EROD-inducing capacity of HS, three-spined sticklebacks (Gasterosteus aculeatus) were exposed to HS of natural or synthetic origin. Both kinds of HS caused significant EROD induction. Gill EROD activities were also induced in sticklebacks exposed to ethynylestradiol (EE2) and β-naphthoflavone (βNF), alone and in combinations. Production of vitellogenin was induced in sticklebacks exposed to ≥50 ng EE2/l and a significant decrease in spiggin production was observed in individuals exposed to 170 ng EE2/l.

Results from this thesis further strengthen the contention that gill EROD activity is a very sensitive biomarker for CYP1A inducers and that the stickleback is a suitable biomonitoring species, especially for exposure to CYP1A inducers. The finding that not only classical CYP1A inducers but also HS and high EE2 concentrations stimulate gill EROD activity is of significance for the interpretation of biomonitoring data.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2007. 59 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 345
Keyword
Biology, three-spined stickleback, biomarker, EROD, ethynylestradiol, gill, vitellogenin, spiggin, CYP1A inducers, humic substances, sperm quality, biomonitoring, Biologi
Identifiers
urn:nbn:se:uu:diva-8222 (URN)978-91-554-6975-7 (ISBN)
Public defence
2007-10-19, Lindahlsalen, EBC, Norbyvägen 18A, Uppsala, 09:00 (English)
Opponent
Supervisors
Available from: 2007-09-28 Created: 2007-09-28 Last updated: 2011-01-27Bibliographically approved

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