Non-hybridisation saturable mechanisms play a role in the uptake of 68Ga-labelled LNA-DNA mixmer antisense oligonucleotides in rats
2009 (English)In: Oligonucleotides, ISSN 1545-4576 (Print), Vol. 19, no 3, 223-231 p.Article in journal (Refereed) Published
Oligonucleotides (ODN) are key molecules for the aim of preventing translation of a gene product or monitoring gene expression in tissues. However, multiple methodological and biological hurdles need to be solved before in vivo application in humans will be possible. For positron emission tomography (PET) investigations, a 20-mer DNA-locked nucleic acid (LNA) mixmer ODN specifi c for rat chromogranin-A mRNA was labeled with Ga-68 and its uptake was examined in vivo in rats with and without blocking of scavenger receptors by polyribo-nucleotides. In addition, uptake studies of Ga-68-LNA were performed with respect to time and concentration in human and rat cell lines. The human cell lines did not express the target mRNA. Both polyinosinic acid (poly-I) and polyadenylic acid (poly-A) reduced the uptake in rat tissues and in human cell lines. Poly-I was found to be more effective in the liver whereas poly-A was more effective in the kidney. In addition, the blockade by poly-I was statistically significant in the pancreas, adrenal gland, bone marrow, intestine, testis, urinary bladder, muscle, parotid gland, and heart, whereas poly-A also caused significant reduction in pancreas, adrenal gland, and bone marrow but not as much as in kidney. Cell culture study showed a 2-phase dose-dependent uptake characteristic with a saturable and a passive diffusion-like phase; however, these 2 phases were not so well expressed in the rat cell line. The results suggest that scavenger receptors or other saturable processes unrelated to hybridization may be involved in the tissue uptake of Ga-68-LNA and in the clearance of antisense ODN through the liver, kidney, spleen, and bone marrow. The fact that these processes may be sequence-dependent suggests that proof of in vivo hybridization through imaging may not be obtained by only comparing sense and antisense sequences and proving dose-dependency.
Place, publisher, year, edition, pages
2009. Vol. 19, no 3, 223-231 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-96301DOI: 10.1089/oli.2009.0192ISI: 000270785700002OAI: oai:DiVA.org:uu-96301DiVA: diva2:170831