Detection of antibiotic resistance is paramount for accurate and effective treatment of infectious bacterial diseases. There is a growing interest for methods making it possible to identify the specific genes giving rise to antibiotic resistance. Molecular detection approaches have evolved to the point where many techniques can be performed in clinics, bedside setups or implemented in microfluidic chips as they require little equipment and training. Rolling circle amplification (RCA) is an isothermal DNA amplification technique that amplifies synthetic circular DNA probes that have been hybridized to a complementary target present in the sample. To increase the number of amplification products, several rounds of RCA may be performed on a single sample. This technique is denoted as circle-to-circle amplification (C2CA).