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Quantitative proteomic comparison of mouse peroxisomes from liver and kidney
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
2007 (English)In: Proteomics, ISSN 1615-9853, E-ISSN 1615-9861, Vol. 7, no 11, 1916-1928 p.Article in journal (Refereed) Published
Abstract [en]

The peroxisome plays a central role in the catabolic and anabolic pathways that contribute to the lipid homeostasis. Besides this main function, this organelle has gained functional diversity. Although several approaches have been used for peroxisomal proteome analysis, a quantitative protein expression analysis of peroxisomes from different tissues has not been elucidated yet. Here, we applied a 2-DE-based method on mouse liver and kidney peroxisomal enriched fractions to study the tissue-dependent protein expression. Ninety-one spots were identified from the 2-DE maps from pH 3.0-10.0 and 51 spots from the basic range corresponding to 31 peroxisomal proteins, 10 putative peroxisomal, 6 cytosolic, 17 mitochondrial and 1 protein from endoplasmic reticulum. Based on the identification and on the equivalent quality of both tissue preparations, the differences emerging from the comparison could be quantified. In liver, proteins involved in pathways such as α- and β-oxidation, isoprenoid biosynthesis, amino acid metabolism and purine and pirimidine metabolism were more abundant whereas in kidney, proteins from the straight-chain fatty acid β-oxidation were highly expressed. These results indicate that tissue-specific functional classes of peroxisomal proteins could be relevant to study peroxisomal cellular responses or pathologies. Finally, a web-based peroxisomal proteomic database was built.

Place, publisher, year, edition, pages
2007. Vol. 7, no 11, 1916-1928 p.
Keyword [en]
2-DE, Kidney, Liver, Peroxisome, Proteomic comparison
National Category
Biological Sciences
URN: urn:nbn:se:uu:diva-96430DOI: 10.1002/pmic.200600638ISI: 000247325800017PubMedID: 17474143OAI: oai:DiVA.org:uu-96430DiVA: diva2:171001
Available from: 2007-11-15 Created: 2007-11-15 Last updated: 2011-04-12Bibliographically approved
In thesis
1. Proteomic Analysis of Peroxisomal Proteins
Open this publication in new window or tab >>Proteomic Analysis of Peroxisomal Proteins
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Peroxisome is a ubiquitous eukaryotic organelle with a single-layer membrane. It maintains various functions that differ depending on the species and cell types, as well as the environmental or developmental conditions.

In the first part of this thesis, the peroxisomal protein content was systematically analyzed in different organs in mouse from different ages using proteomic approaches. Thirty-one peroxisomal proteins were identified and ten putative peroxisomal proteins were suggested. The results indicate that peroxisomal proteins show a tissue-specific functional response to the aging process that is probably dependent on their differential regeneration capacity. Besides, alteration in the fatty acid metabolism could alter membrane protein functions; decrease in catalase expression in kidney may contribute to oxidative stress and isoprenoid biosynthesis could contribute to decline in bile salt synthesis. The ability to detect changes in the peroxisomal proteome associated with organ impairment during the course of aging would provide a conceptual framework to understand the role of peroxisome in aging.

In the second part, peroxisome proteomics was used as a novel approach in marine pollution assessment. The peroxisomal protein expression profiles were obtained and identified from mussel Mytilus sp. exposed to different pollutants, in both laboratory and field experiments. The identified proteins were involved in α- and β–oxidation pathways, xenobiotics and amino acid metabolism, cell signalling, oxyradical metabolism, peroxisomal assembly, respiration and cytoskeleton pathway, etc. Generally, these findings suggest that protein expression signatures could become a valuable tool to monitor the presence of pollutants in marine environment.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2007. 54 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 365
Cell and molecular biology, peroxisome, proteomics, biomarker, aging, pollution assessment, Cell- och molekylärbiologi
urn:nbn:se:uu:diva-8307 (URN)978-91-554-7020-3 (ISBN)
Public defence
2007-12-08, C10:305, Biomedical Center, Husarg. 3, Uppsala, 10:00
Available from: 2007-11-15 Created: 2007-11-15Bibliographically approved

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