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Peroxisomal proteomic approach for protein profiling in blue mussels (Mytilus edulis) exposed to crude oil
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
2007 (English)In: Biomarkers, ISSN 1354-750X, Vol. 12, no 1, 47-60 p.Article in journal (Refereed) Published
Abstract [en]

Peroxisomal proteomic protein profiles of exposure to marine pollution have been recently introduced in biomonitoring experiments. However, laboratory experiments to study the independent effect of common pollutants are needed to define a minimal protein expression signature (PES) of exposure to a specific pollutant. The aim of this study was to obtain PESs in blue mussels (Mytilus edulis) exposed to two different crude oil mixtures for future application in biomonitoring areas affected by oil spills. In the study, peroxisome-enriched fractions from digestive gland of M. edulis (L., 1758) were analysed by two-dimensional fluorescence difference electrophoresis (DIGE) and mass spectrometry (MS) after 3 weeks of exposure to crude oil mixtures: crude oil or crude oil spiked with alkylated phenols (AP) and extra polycyclic aromatic hydrocarbons (PAH) in a laboratory flow-through system. A minimal PES composed by 13 protein spots and unique PESs of exposure to the two different mixtures were identified. A total of 22 spots from the two-dimensional maps that had shown a significant increase or decrease in abundance in each of the exposed groups exposed were analysed. The hierarchical clustering analysis succeeded in discriminating the exposed groups from the control groups based on the unique PES. The PESs obtained were consistent with protein patterns obtained in previous field experiments. The results suggest that the protein profiles obtained by peroxisomal proteomics could be used to assess oil exposure in marine pollution assessments.

Place, publisher, year, edition, pages
2007. Vol. 12, no 1, 47-60 p.
National Category
Biological Sciences
URN: urn:nbn:se:uu:diva-96433DOI: 10.1080/13547500600943528ISI: 000243660200004OAI: oai:DiVA.org:uu-96433DiVA: diva2:171004
Available from: 2007-11-15 Created: 2007-11-15 Last updated: 2011-02-16Bibliographically approved
In thesis
1. Proteomic Analysis of Peroxisomal Proteins
Open this publication in new window or tab >>Proteomic Analysis of Peroxisomal Proteins
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Peroxisome is a ubiquitous eukaryotic organelle with a single-layer membrane. It maintains various functions that differ depending on the species and cell types, as well as the environmental or developmental conditions.

In the first part of this thesis, the peroxisomal protein content was systematically analyzed in different organs in mouse from different ages using proteomic approaches. Thirty-one peroxisomal proteins were identified and ten putative peroxisomal proteins were suggested. The results indicate that peroxisomal proteins show a tissue-specific functional response to the aging process that is probably dependent on their differential regeneration capacity. Besides, alteration in the fatty acid metabolism could alter membrane protein functions; decrease in catalase expression in kidney may contribute to oxidative stress and isoprenoid biosynthesis could contribute to decline in bile salt synthesis. The ability to detect changes in the peroxisomal proteome associated with organ impairment during the course of aging would provide a conceptual framework to understand the role of peroxisome in aging.

In the second part, peroxisome proteomics was used as a novel approach in marine pollution assessment. The peroxisomal protein expression profiles were obtained and identified from mussel Mytilus sp. exposed to different pollutants, in both laboratory and field experiments. The identified proteins were involved in α- and β–oxidation pathways, xenobiotics and amino acid metabolism, cell signalling, oxyradical metabolism, peroxisomal assembly, respiration and cytoskeleton pathway, etc. Generally, these findings suggest that protein expression signatures could become a valuable tool to monitor the presence of pollutants in marine environment.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2007. 54 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 365
Cell and molecular biology, peroxisome, proteomics, biomarker, aging, pollution assessment, Cell- och molekylärbiologi
urn:nbn:se:uu:diva-8307 (URN)978-91-554-7020-3 (ISBN)
Public defence
2007-12-08, C10:305, Biomedical Center, Husarg. 3, Uppsala, 10:00
Available from: 2007-11-15 Created: 2007-11-15Bibliographically approved

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