Expression of Markers of Activity in Cultured Human Osteoblasts: Effects of Interleukin-4 and Interleukin-13
2010 (English)In: Scandinavian Journal of Clinical and Laboratory Investigation, ISSN 0036-5513, Vol. 70, no 5, 338-342 p.Article in journal (Refereed) Published
Cytokines regulate proliferation, differentiation and activation of osteoblasts. Interleukin-4 (IL-4) and interleukin-13 (IL-13) takes part in this regulation by inhibiting proliferation and by enhancement of interleukin-6 (IL-6) formation in cultured human osteoblasts (hOBs). In the present study we have investigated the effects of IL-4 and IL-13 on markers of osteoblastic activity in isolated hOBs. Treatment with either IL-4 or IL-13 (1–100 pM) stimulated the formation of alkaline phosphatase (ALP) dose-dependently, detected by enzyme reaction and histochemistry. IL-4 and IL-13 also induced an increase in the secretion of procollagen type I carboxypeptide (PICP) from cultured hOBs, measured by RIA. Osteocalcin secretion measured by ELISA-technique was unaffected. The rate of mineralization, assessed by von Kossa and Alizarin Red staining, was clearly enhanced in hOBs stimulated by IL-4 or IL-13. In conclusion IL-4 and IL-13 exert multiple effects on osteoblast activity in cultured hOBs. Stimulation of ALP secretion together with enhanced collagen secretion and mineralization suggests that IL-4 and IL-13 also have the capacity to maintain hOBs in a differentiated, productive phase.
Place, publisher, year, edition, pages
2010. Vol. 70, no 5, 338-342 p.
Alkaline phosphatase, osteoblasts, collagen type I, cell differentiation, bone mineralization, osteocalcin
Research subject Orthopaedics
IdentifiersURN: urn:nbn:se:uu:diva-96654DOI: 10.3109/00365513.2010.488698ISI: 000282186000006OAI: oai:DiVA.org:uu-96654DiVA: diva2:171301