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Secondary chemistry and ribosomal DNA data congruencies in Arnica (Asteraceae)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Division of Pharmacognosy. Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Evolutionary Biology, Systematic Botany.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Division of Pharmacognosy.
Albert-Ludwigs-Universität, Freiburg, Germany.
Albert-Ludwigs-Universität, Freiburg, Germany.
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2009 (English)In: Cladistics, ISSN 0748-3007, E-ISSN 1096-0031, Vol. 25, no 1, 78-92 p.Article in journal (Refereed) Published
Abstract [en]

To investigate possible congruencies between DNA sequence data and secondary chemistry, we compared nuclear ribosomal DNA (nrDNA) sequence data, sesquiterpene lactone (STL) contents, and cytometric data from 35 accessions of 16 Arnica (Asteraceae) species and two outgroup taxa (Layia hieracioides and Madia sativa), using phylogenetic inference and principal component analysis (PCA). Several groups supporting multiple accessions of the same species (of A. montana, A. longifolia, A. gracilis, and A. chamissonis) are congruent between the phylogenetic trees based on nrDNA and STL data. Sesquiterpene lactone profiles were found to be highly consistent within multiple samples of A. montana and A. longifolia respectively. Moreover, sesquiterpene lactone data support subspecies classifications of A. chamissonis and A. parryi, with additional support from DNA sequence data and cytometric data. Morphology, STL data (PCA), cytometric data and DNA sequence data suggest a hybrid origin of one accession (A. gracilis × longifolia). In A. gracilis, A. latifolia, and Layia hieracioides, previously not investigated for STLs, we found large amounts of xanthalongin derivatives. This is the first time STLs have been reported from subtribe Madiinae.

Place, publisher, year, edition, pages
2009. Vol. 25, no 1, 78-92 p.
National Category
Pharmaceutical Sciences Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-96740DOI: 10.1111/j.1096-0031.2008.00244.xISI: 000262636700004OAI: oai:DiVA.org:uu-96740DiVA: diva2:171416
Available from: 2008-02-21 Created: 2008-02-21 Last updated: 2017-12-14Bibliographically approved
In thesis
1. Phylogenies and Secondary Chemistry in Arnica (Asteraceae)
Open this publication in new window or tab >>Phylogenies and Secondary Chemistry in Arnica (Asteraceae)
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The genus Arnica (Asteraceae) was investigated for phylogenetic relationships and sesquiterpene lactone (STL) content with the aims to trace the evolutionary history of the genus and to investigate possible congruencies between DNA sequence data, secondary chemistry, and biological activity.

Complex evolutionary patterns in Arnica are evident from phylogenetic analyses of chloroplast regions (the rpl16 and rps16 introns and the psbA–trnH, ycf4–cemA, and trnT–L spacers), nuclear ribosomal regions (the internal and external transcribed spacers) and the nuclear low-copy DNA region coding for the second largest subunit of RNA polymerase II (RPB2) between exons 17 and 23. Polymorphism was detected in nuclear ribosomal and low-copy regions, likely caused by polyploidy and agamospermy. Lineage sorting and/or hybridization is a possible explanation for incongruencies between topologies of the different DNA regions. None of the five subgenera in Arnica constitute a monophyletic group according to any of our analyses.

Sesquiterpene lactone profiles were compared to nuclear ribosomal DNA data using phylogenetic inference and principal component analysis for 33 accessions of 16 species. Clusters supported by both STL chemistry and ribosomal DNA sequence data consist of multiple accessions of the same species (e.g. A montana and A. longifolia), indicating that these species are well defined both genetically and chemically, based on our sampling. Support for subspecies classification of A. chamissonis and A. parryi was found in chemical data. For the first time STLs are reported from subtribe Madiinae, sister to Arniciinae.

Anti-inflammatory properties, as measured by inhibition of human neutrophil elastase release from neutrophils and inhibition of the binding of transcription factor NF-κB to DNA, were investigated for extracts of 12 Arnica species. Arnica montana, A. chamissonis and A. longifolia accessions show high inhibitory effects in both bioassays. Generally, species with a more diverse STL chemistry also possess the strongest inhibitory activity in the bioassays.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2008. 57 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 392
Keyword
Biology, phylogeny, ITS, ETS, RPB2, sesquiterpene lactones, PCA, bioassay, NF-κB, neutrophil, chloroplast, Biologi, Arnica, Asteraceae
Identifiers
urn:nbn:se:uu:diva-8459 (URN)978-91-554-7092-0 (ISBN)
Public defence
2008-03-14, Lindahlsalen, EBC, Norbyvägen 18A, Uppsala, 10:00
Opponent
Supervisors
Available from: 2008-02-21 Created: 2008-02-21Bibliographically approved

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Backlund, AndersAndreasen, Katarina

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