Single molecule analysis of combinatorial splicing
2010 (English)In: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 38, no 16, e163- p.Article in journal (Refereed) Published
Alternative splicing forms diverse mRNA isoform populations from a single ancestral pre-mRNA and thereby enhances complexity of transcript structure and of gene function. We describe a method called spliceotyping, which translates combinatorial mRNA splicing patterns into a library of binary strings of nucleic acid tags, encoding the exon composition of transcripts. The transcript abundance is registered by counts of individual molecules and individual exon inclusion patterns are represented as strings of binary data.
The technique is illustrated by analyzing the splicing patterns of the adenovirus early 1A gene and the beta actin reference transcript. The method permits different genes to be analyzed in parallel and will be valuable for elucidating the complex effects of combinatorial splicing.
Place, publisher, year, edition, pages
2010. Vol. 38, no 16, e163- p.
Splicing, Combinatorial, RCA, Single molecule, Amplified Single Molecule Detection
Medical and Health Sciences
Research subject Molecular Medicine
IdentifiersURN: urn:nbn:se:uu:diva-96780DOI: 10.1093/nar/gkq581ISI: 000281720500003PubMedID: 20587504OAI: oai:DiVA.org:uu-96780DiVA: diva2:171466