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Initial evaluation of biotinylated bilayer disks as model membranes for biosensor analyses
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Physical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Biochemistry and Organic Chemistry.
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(English)Manuscript (Other (popular science, discussion, etc.))
Identifiers
URN: urn:nbn:se:uu:diva-96810OAI: oai:DiVA.org:uu-96810DiVA: diva2:171507
Available from: 2008-03-06 Created: 2008-03-06 Last updated: 2011-03-21
In thesis
1. Nanosized Bilayer Disks as Model Membranes for Interaction Studies
Open this publication in new window or tab >>Nanosized Bilayer Disks as Model Membranes for Interaction Studies
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

PEG-lipid stabilized bilayer disks have been found in lipid mixtures containing polyethylene glycol (PEG)-lipids where the combination of a high bending rigidity and low PEG-lipid/lipid miscibility favours disk formation. The disks are planar and circular in shape and their long-term stability is excellent. Theoretical calculations and experimental observations suggest that the micelle forming PEG-lipid are situated at the rim of the aggregate, protecting the hydrophobic lipid chains in the bulk of the aggregate from contact with water. This thesis deals with fundamental aspects concerning the lipid distribution in the disks, as well as with development, optimization, and initial evaluation of the disks as model membranes in partition and interaction studies.

Small angle neutron scattering was used to study the partial segregation of components within the bilayer disk. The experiments verified that the PEG-lipids segregate and accumulate at the bilayer disk rim. The proof of component segregation is important from a fundamental point of view and useful, as exemplified in the below-mentioned study of melittin-lipid interaction, when interpreting partition or binding data obtained from studies based on bilayer disks.

Today liposomes are often used as model membranes in partition and interaction studies. Using liposomes to predict, e.g., drug partitioning can however have certain drawbacks. In this thesis the disks were proven to be attractive alternatives to liposomes as model membranes in partition studies. The formation of bilayer disks by a technique based on detergent depletion enabled incorporation of a transmembrane protein in the bilayer disks and opened up for the use of disks as model membranes in membrane protein studies. Further, bilayer disks were used in a comparative study focused on the effect of aggregate curvature on the binding of the peptide melittin. Various techniques were used to perform initial evaluations of the bilayer disks as model membranes. Of these, capillary electrophoresis and biosensor-based technology had not been used before in combination with bilayer disks.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2008. 58 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 400
Keyword
Disk, disc, lipid bilayer, PEG-lipid, model membrane, interaction, partitioning, drug, liposome, cryo-TEM, neutron scattering, capillary electrophoresis, immobilization, biosensor, membrane protein, melittin
National Category
Chemical Sciences
Identifiers
urn:nbn:se:uu:diva-8495 (URN)978-91-554-7109-5 (ISBN)
Public defence
2008-03-28, B22, BMC, Husargatan 3, Uppsala, 10:15 (English)
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Supervisors
Available from: 2008-03-06 Created: 2008-03-06 Last updated: 2010-03-04Bibliographically approved

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Danielson, HelenaEdwards, Katarina

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