uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Straightforward and rapid determination of sulfadoxine and sulfamethoxazole in capillary blood on sampling paper with liquid chromatography and UV detection
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Analytical Chemistry.
2009 (English)In: Transactions of the Royal Society of Tropical Medicine and Hygiene, ISSN 0035-9203, E-ISSN 1878-3503, Vol. 103, no 4, p. 371-376Article in journal (Refereed) Published
Abstract [en]

A method for the determination of sulfadoxine and sulfamethoxazole in capillary blood on sampling paper has been developed and validated. The method is straightforward with minimal sample preparation, and is suitable for rural settings. Separation of sulfadoxine, sulfamethoxazole and internal standard was performed using a Purospher STAR RP-18 endcapped LC column (150×4.6mm) with a mobile phase consisting of acetonitrile:sodium acetate buffer pH 5.2, I=0.1 (1:2, v/v). For sulfadoxine, the within-day precision was 5.3% at 15μmol/l and 3.7% at 600μmol/l, while for sulfamethoxazole it was 5.7% at 15μmol/l and 3.8% at 600μmol/l. The lower limit of quantification was determined to 5μmol/l and precision was 5.5% and 5.0% for sulfadoxine and sulfamethoxazole, respectively.

Place, publisher, year, edition, pages
2009. Vol. 103, no 4, p. 371-376
Keyword [en]
Malaria, Pneumonia, Sulfadoxine, Sulfamethoxazole, Chromatography, Dried blood spots
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-96865DOI: 10.1016/j.trstmh.2008.11.031ISI: 000264976700009OAI: oai:DiVA.org:uu-96865DiVA: diva2:171589
Available from: 2008-03-19 Created: 2008-03-19 Last updated: 2017-12-14Bibliographically approved
In thesis
1. Drug Analysis: Bioanalytical Method Development and Validation
Open this publication in new window or tab >>Drug Analysis: Bioanalytical Method Development and Validation
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis describes bioanalytical methods for drug determination in biological matrixes, with drugs in focus used against diseases largely affecting low-income countries.

Solid-phase extraction is used for sample cleanup, and processed samples are analyzed by liquid chromatography. Developed bioanalytical methods are validated according to international guidelines.

Eflornithine (DFMO) is a chiral drug, used for treating human African trypanosomiasis. A bioanalytical method for determination of DFMO enantiomers in plasma is presented. The enantiomers are detected by evaporative light-scattering detection. The method has been applied to determination of D-DFMO and L-DFMO in rats, after intravenous and oral administration of racemic DFMO. It is concluded that DFMO exhibits enantioselective absorption, with the more potent enantiomer L-DFMO being less favored.

Sulfadoxine (SD) and sulfamethoxazole (SM) are sulfa-drugs used for malaria and pneumonia respectively. Two methods are described for simultaneous determination of SD and SM in capillary blood sampled on filter paper. The former method allows direct injection of extracts from dried blood spots (DBS), while for the latter method solid-phase extraction is added. Pre-analytical factors contributing to measurement uncertainty is also discussed, and it is concluded that it is of high importance that homogeneity in type of sampling paper and sampling volume is assured.

Piperaquine (PQ) is an antimalarial, increasingly used in artemisinin combination therapy. A method for determination of piperaquine in DBS is presented. By using a monolithic LC column, a very short LC analysis of two minutes per sample is achieved.

A method for simultaneous determination of three antiretroviral drugs i.e. lamivudine (3TC), zidovudine (AZT) and nevirapine (NVP), in DBS samples is described. The method is applied to drug determination in two subjects after receiving standard antiretroviral treatment. Conclusion is that the method is suitable for determination of 3TC and NVP, and to some extent for AZT.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2008. p. 59
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 406
Keyword
Analytical chemistry, African trypanosomiasis, calibration model, chiral chromatography, dried blood spots, eflornithine, evaporative light-scattering detection, HIV/AIDS, lamivudine, liquid chromatography, malaria, nevirapine, piperaquine, pneumonia, solid-phase extraction, sulfadoxine, sulfamethoxazole, validation, zidovudine, Analytisk kemi
Identifiers
urn:nbn:se:uu:diva-8547 (URN)978-91-554-7125-5 (ISBN)
Public defence
2008-04-18, Clas Ohlson room, Tenoren, Högskolan Dalarna, Borlänge, 13:00 (English)
Opponent
Supervisors
Available from: 2008-03-19 Created: 2008-03-19 Last updated: 2009-03-24Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full text
By organisation
Analytical Chemistry
In the same journal
Transactions of the Royal Society of Tropical Medicine and Hygiene
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 932 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf