Straightforward and rapid determination of sulfadoxine and sulfamethoxazole in capillary blood on sampling paper with liquid chromatography and UV detection
2009 (English)In: Transactions of the Royal Society of Tropical Medicine and Hygiene, ISSN 0035-9203, E-ISSN 1878-3503, Vol. 103, no 4, 371-376 p.Article in journal (Refereed) Published
A method for the determination of sulfadoxine and sulfamethoxazole in capillary blood on sampling paper has been developed and validated. The method is straightforward with minimal sample preparation, and is suitable for rural settings. Separation of sulfadoxine, sulfamethoxazole and internal standard was performed using a Purospher STAR RP-18 endcapped LC column (150×4.6mm) with a mobile phase consisting of acetonitrile:sodium acetate buffer pH 5.2, I=0.1 (1:2, v/v). For sulfadoxine, the within-day precision was 5.3% at 15μmol/l and 3.7% at 600μmol/l, while for sulfamethoxazole it was 5.7% at 15μmol/l and 3.8% at 600μmol/l. The lower limit of quantification was determined to 5μmol/l and precision was 5.5% and 5.0% for sulfadoxine and sulfamethoxazole, respectively.
Place, publisher, year, edition, pages
2009. Vol. 103, no 4, 371-376 p.
Malaria, Pneumonia, Sulfadoxine, Sulfamethoxazole, Chromatography, Dried blood spots
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-96865DOI: 10.1016/j.trstmh.2008.11.031ISI: 000264976700009OAI: oai:DiVA.org:uu-96865DiVA: diva2:171589