A great number of chemicals - many of which are hazardous to human health and the environment - are used along the complex supply chains of textiles. These complexities and hazards make knowledge for understanding chemical properties and management practices at multiple nodes central to the responsible management of textile supply chains. This study investigates the knowledge requirements of, and the knowledge strategies used by, textile procuring organisations in response to both external stakeholders and internal pressure. Based on a qualitative study of small and medium-sized Swedish textile procuring organisations, the paper describes these knowledge challenges and analyses how efforts to meet them relate to expressed commitments and capabilities for responsible supply chain management (RSCM), as well as to organisational characteristics (i.e. whether the organisation is private or public, small or large, and whether textiles is a core or peripheral activity). It was found that several textile procuring organisations expressed commitment towards achieving RSCM. However, most organisations felt that they lacked capacity to rise to the challenge. There was a poor state of knowledge regarding many chemical substances and inherent difficulties in gaining knowledge of chemical risks and how to manage these. Moreover, the input of knowledge was limited and based on only a few key sources. Such factors proved to be obstacles for procuring organisations when attempting to facilitate responsible management upstream in complex supply chains.