Development of the Tracer-Pulse method for adsorption studies of analyte mixures in liquid chromatography utilizing mass spectrometric detection
2008 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 80, no 6, 2105-2112 p.Article in journal (Refereed) Published
The tracer-pulse method provides the real adsorption data points directly from simple, straightforward calculations and is therefore a superior method for multicomponent adsorption isotherm determination in HPLC. Only one important problem has restricted its use so far: the tracer peaks are invisible using any conventional detection principle. We present a solution to this problem with an approach with a firm base in analytical chemistry, utilizing stable isotopes and mass spectrometric detection. The new approach was used for the determination of binary adsorption isotherms, and a systematic investigation was made of its main sources of error. With this modification, the tracer method can be a prime choice for future characterizations of multicomponent separation systems and of competitive drug binding studies.
Place, publisher, year, edition, pages
2008. Vol. 80, no 6, 2105-2112 p.
IdentifiersURN: urn:nbn:se:uu:diva-96956DOI: 10.1021/ac702399aISI: 000254017000035PubMedID: 18290673OAI: oai:DiVA.org:uu-96956DiVA: diva2:171704