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Steroid profiles in ovarian follicular fluid samples using high sensitivity tandem mass spectrometry methods
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
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In: Article in journal (Refereed) Submitted
Identifiers
URN: urn:nbn:se:uu:diva-97095OAI: oai:DiVA.org:uu-97095DiVA: diva2:171884
Available from: 2008-04-22 Created: 2008-04-22 Last updated: 2011-03-21
In thesis
1. Mass Spectrometric Applications for Diagnosing Metabolic and Endocrine Diseases
Open this publication in new window or tab >>Mass Spectrometric Applications for Diagnosing Metabolic and Endocrine Diseases
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Disease-specific compounds (biomarkers) are analyzed in clinical laboratories to assist with diagnosing diseases. This thesis describes development and validation of liquid chromatography tandem mass spectrometry (LC-MS/MS) based tests for diagnosing a diverse group of endocrine and metabolic diseases. The analytical methods used on-line and off-line sample extraction and analytical derivatization as means of enhancing the analytical sensitivity, specificity and clinical utility. All developed methods were extensively validated and reference intervals for the biomarker concentrations were established in blood samples of healthy adults and children. Advantages of the LC-MS/MS as an analytical technique include possibility of simultaneous measurement of multiple analytes and ability of confirming their identity. In this thesis we proposed and evaluated approaches for the assessment of the specificity of analysis in the methods that use tandem mass spectrometry detection. To enhance throughput of the LC-MS/MS tests for the biomarkers that have endogenous or exogenous isomers an approach was developed for quantitation of isomers from unresolved chromatographic peaks. Using methods developed in this thesis we performed a study of the steroidogenesis in ovarian follicles of healthy women and women with polycystic ovary syndrome (PCOS). Obtained data on the steroid concentrations and associations between the steroid metabolites in the pathway would be helpful for better understanding of the ovarian pathophysiology. Potential biomarkers of PCOS were identified in the thesis; further studies will be necessary to confirm their clinical utility.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2008. 80 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 426
Keyword
Analytical chemistry, biomarker, liquid chromatography, tandem mass spectrometry, LC-MS/MS, sample preparation, isomers, steroid hormones, congenital adrenal hyperplasia, polycystic ovary syndrome, Analytisk kemi
Identifiers
urn:nbn:se:uu:diva-8658 (URN)978-91-554-7172-9 (ISBN)
Public defence
2008-05-16, BMC B:41, BMC, Husargatan 3, SE-751 24, Uppsala, Sweden, 10:15
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Available from: 2008-04-22 Created: 2008-04-22 Last updated: 2011-05-10Bibliographically approved

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Bergquist, Jonas

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