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Identification of the novel melanocytic markers Discs large homolog 5 and Syntaxin-7 in benign melanocytic lesions and malignant melanoma
Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Genetics and Pathology.
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Article in journal (Refereed) Submitted
Identifiers
URN: urn:nbn:se:uu:diva-97145OAI: oai:DiVA.org:uu-97145DiVA: diva2:171953
Available from: 2008-04-25 Created: 2008-04-25Bibliographically approved
In thesis
1. Antibody-based Profiling of Expression Patterns using Cell and Tissue Microarrays
Open this publication in new window or tab >>Antibody-based Profiling of Expression Patterns using Cell and Tissue Microarrays
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In this thesis, methods to study gene and protein expression in cells and tissues were developed and utilized in combination with protein-specific antibodies, with the overall objective to attain greater understanding of protein function.

To analyze protein expression in in vitro cultured cell lines, a cell microarray (CMA) was developed, facilitating antibody-based protein profiling of cell lines using immunohistochemistry (IHC). Staining patterns in cell lines were analyzed using image analysis, developed to automatically identify cells and immunohistochemical staining, providing qualitative and quantitative measurements of protein expression. Quantitative IHC data from CMAs stained with nearly 3000 antibodies was used to evaluate the adequacy of using cell lines as models for cancer tissue. We found that cell lines are homogenous with respect to protein expression profiles, and generally more alike each other, than corresponding cancer cells in vivo. However, we found variability between cell lines in regards to the level of retained tumor phenotypic traits, and identified cell lines with a preserved link to corresponding cancer, suggesting that some cell lines are appropriate model systems for specific tumor types.

Specific gene expression patterns were analyzed in vitiligo vulgaris and malignant melanoma. Transcriptional profiling of vitiligo melanocytes revealed dysregulation of genes involved in melanin biosynthesis and melanosome function, thus highlighting some mechanisms possibly involved in the pathogenesis of vitiligo. Two new potential markers for infiltrating malignant melanoma, Syntaxin-7 and Discs large homolog 5, were identified using antibody-based protein profiling of melanoma in a tissue microarray format. Both proteins were expressed with high specificity in melanocytic lesions, and loss of Syntaxin-7 expression was associated with more high-grade malignant melanomas.

In conclusion, the combination of antibody-based proteomics and microarray technology provided valuable information of expression patterns in cells and tissues, which can be used to better understand associations between protein signatures and disease.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2008. 51 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 344
Keyword
Pathology, antibody-based proteomics, tissue microarray, cell line, immunohistochemistry, melanocytes, image analysis, biomarker, Patologi
Identifiers
urn:nbn:se:uu:diva-8680 (URN)978-91-554-7183-5 (ISBN)
Public defence
2008-05-16, Rudbecksalen, Rudbecklaboratoriet, Dag Hammarskjöldsväg 20, Uppsala, 10:00
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Available from: 2008-04-25 Created: 2008-04-25Bibliographically approved

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