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Determination of Salbutamol in Tablets by Multiple-injection Capillary Zone Electrophoresis
Uppsala University, Medicinska vetenskapsområdet, Faculty of Pharmacy, Department of Medicinal Chemistry, Analytical Pharmaceutical Chemistry.
Manuscript (Other academic)
URN: urn:nbn:se:uu:diva-97252OAI: oai:DiVA.org:uu-97252DiVA: diva2:172109
Available from: 2008-05-14 Created: 2008-05-14 Last updated: 2010-01-13Bibliographically approved
In thesis
1. Separation of Pharmaceuticals by Capillary Electrophoresis using Partial Filling and Multiple-injections
Open this publication in new window or tab >>Separation of Pharmaceuticals by Capillary Electrophoresis using Partial Filling and Multiple-injections
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Different multiple-injection methodologies and the partial filling technique (PFT) have been utilized for separation of pharmaceuticals by capillary elec-trophoresis.

In multiple-injection capillary zone electrophoresis (MICZE), the samples and all standards, used for construction of the calibration curve, are analyzed within a single run. Four different modes of MICZE have been described by means of equations, which were experimentally verified. The developed equations facilitate the transfer from conventional single-injection CZE to one or more of these MICZE-modes, depending on the selectivity between the analyte and the injection marker. The applicability of two of these modes was then demonstrated by quantification of buserelin and salbutamol, re-spectively in commercially available pharmaceutical products. The content of buserelin in an injection solution was determined to 0.94 mg/ml, which only deviated slightly from the declared concentration (1 mg/ml). An alter-native mode of MICZE, offering a higher number of sequential sample injec-tions, was then utilized for single-run determination of salbutamol in 15 tab-lets, with a labelled content of 8 mg. The average content of the tablets was determined to 7.8 mg, with an intra-tablet variation of 3 % or less.

Moreover, UV- and mass-spectrometric detection of enantiomeric amines, resolved by non-aqueous capillary electrophoresis (NACE), was demon-strated. Separation of enantiomeric amines was achieved using the chiral selector (-)-2,3:4,6-di-O-isopropylidene-2-keto-L-gulonic acid, (-)-DIKGA. Introduction of the non-volatile (-)-DIKGA into the mass-spectrometer was avoided by using the PFT, where the capillary is only partially filled with electrolyte containing the chiral selector.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2008. 60 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Pharmacy, ISSN 1651-6192 ; 75
Capillary Coating, Chiral Separation, Enantiomers, Mass-spectrometric Detection, Method Validation, Multiple-injection Capillary Zone Electrophoresis, Non-aqueous Capillary Electrophoresis, Organic Solvents, Pharmaceutical Analysis, Quantitative Analysis
urn:nbn:se:uu:diva-8841 (URN)978-91-554-7211-5 (ISBN)
Public defence
2008-06-05, B41, BMC, Husargatan 3, Uppsala, 13:15
Available from: 2008-05-14 Created: 2008-05-14 Last updated: 2011-01-28Bibliographically approved

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