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Transcription and regulation of the hydrogenase(s) accessory genes, hypFCDEAB, in the cyanobacterium Lyngbya majuscula CCAP 1446/4
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Photochemistry and Molecular Science.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Photochemistry and Molecular Science.
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2007 (English)In: Archives of Microbiology, ISSN 0302-8933, Vol. 188, no 6, 609-617 p.Article in journal (Refereed) Published
Abstract [en]

Lyngbya majuscula CCAP 1446/4 is a filamentous cyanobacterium possessing both an uptake and a bi-directional hydrogenase. The presence of a single copy of the hyp operon in the cyanobacterial genomes suggests that these accessory genes might be responsible for the maturation of both hydrogenases. We investigated the concomitant transcription of hypFCDEAB with the hydrogenases structural genes-hup and hox. RT-PCRs performed with L. majuscula cells grown under different physiological conditions showed a substantial decrease in the relative amount of hupL transcript under non-N-2-fixing conditions. In contrast, no significant differences were observed for the transcript levels of hypFCDEAB in all conditions tested, while minor fluctuations could be discerned for hoxH. Previously, it was demonstrated that the transcriptional regulators NtcA and LexA interact with the promoter regions of hup and hox, respectively, and that putative binding sites for both proteins are present in the hyp promoter of L. majuscula. Therefore, a putative involvement of NtcA and LexA in the regulation of the hyp transcription was investigated. Electrophoretic mobility shift assays resulted in NtcA or LexA-bound retarded fragments, suggesting the involvement of these proteins in the transcriptional regulation of hypFCDEAB.

Place, publisher, year, edition, pages
2007. Vol. 188, no 6, 609-617 p.
Keyword [en]
lyngbya majuscula, hyp, hydrogenase accessory genes, transcriptional regulator factors, NtcA, LexA
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-97275DOI: 10.1007/s00203-007-0281-2ISI: 000251407500007PubMedID: 17639348OAI: oai:DiVA.org:uu-97275DiVA: diva2:172139
Available from: 2008-05-14 Created: 2008-05-14 Last updated: 2012-08-22
In thesis
1. Regulation of the Cyanobacterial Bidirectional Hydrogenase
Open this publication in new window or tab >>Regulation of the Cyanobacterial Bidirectional Hydrogenase
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Today, mankind faces a new challenge in energetic terms: a new Industrial Revolution is imperative, already called by some as an Energetic Revolution. This corresponds to a conversion to clean, environmentally friendly and renewable energy sources. In this context, hydrogen arises as a valid alternative, since its combustion produces a considerable amount of energy and releases solely water as a by-product. In the present thesis, two model cyanobacteria, namely Synechocystis sp. strain PCC 6803 and Anabaena/Nostoc sp. strain PCC 7120, were used to examine the hydrogen metabolism. The efforts were focused on to understand the transcription regulation of the hox genes, encoding the structural elements of the bidirectional hydrogenase enzyme. Here, it is shown that such regulation is operated in a very distinct and intricate way, with different factors contributing to its delicate tuning. While in Synechocystis sp. strain PCC 6803 the hox genes were shown to be transcribed as a single operon, in Anabaena/Nostoc sp. strain PCC 7120 they were shown to be transcribed as two independent operons (possibly three). Two transcription factors, LexA and AbrB-like protein, were identified and further characterized in relation to the hydrogen metabolism. Furthermore, different environmental conditions were demonstrated to operate changes on the transcription of the bidirectional hydrogenase genes. In addition, functional studies of three open reading frames found within the hox operon of Synechocystis sp. strain PCC 6803 suggest that this may be a stress responsive operon. However, based on the gained knowledge, it is still not possible to connect the signal transduction pathways, from the environmental signal, through the response regulator, to the final regulation of the hox genes. Nevertheless, the crucial importance of studying the transcription regulation of the different players involved in the hydrogen metabolism is now established and a new era seems to be rising.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2008. 63 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 437
Keyword
Biology, Biohydrogen, Cyanobacteria, Synechocystis, Anabaena/Nostoc, Bidirectional hydrogenase, Transcription regulation, LexA, AbrB-like protein, Biologi
Identifiers
urn:nbn:se:uu:diva-8862 (URN)978-91-554-7215-3 (ISBN)
Public defence
2008-06-05, Å2001, The Ångström Laboratory, Lägerhyddsvägen 1, Polacksbacken,, Uppsala, 10:15 (English)
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Available from: 2008-05-14 Created: 2008-05-14 Last updated: 2009-08-17Bibliographically approved

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