Histone H2A as a transfection agent in crayfish hematopoietic tissue cells
2007 (English)In: Developmental and Comparative Immunology, ISSN 0145-305X, E-ISSN 1879-0089, Vol. 31, no 4, 340-346 p.Article in journal (Refereed) Published
We report a novel and highly efficient dsRNA transfection system based on one of the nuclear proteins, namely, histone H2A. RT-PCR semi-quantitative analysis of silencing target gene shows that the transfection efficiency of histone H2A is higher than Effectene or liposome-based transfection systems. Importantly, the high efficiency of histone H2A was associated with very low toxicity to the transfected crayfish hematopoietic tissue (Hpt) cells. The non-toxicity, effectiveness and specificity of histone H2A as a transfection agent provides a cheap, simple, highly efficient and reproducible gene delivery system, particularly for the sensitive cell cultures of crustacean animals such as crayfish and shrimp.
Place, publisher, year, edition, pages
2007. Vol. 31, no 4, 340-346 p.
Crayfish, Hematopoietic tissue cells, Histone H2A, RNA interference, Transfection
IdentifiersURN: urn:nbn:se:uu:diva-97409DOI: 10.1016/j.dci.2006.07.007ISI: 000244165900003PubMedID: 16999999OAI: oai:DiVA.org:uu-97409DiVA: diva2:172350