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Phenoloxidase is an important component of the defense against Aeromonas hydrophila infection in a crustacean, Pacifastacus leniusculus
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
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2007 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 282, no 46, 33593-33598 p.Article in journal (Refereed) Published
Abstract [en]

The melanization cascade, in which phenoloxidase is the terminal enzyme, appears to play a key role in recognition of and defense against microbial infections in invertebrates. Here, we show that phenoloxidase activity and melanization are important for the immune defense toward a highly pathogenic bacterium, Aeromonas hydrophila, in the freshwater crayfish, Pacifastacus leniusculus. RNA interference-mediated depletion of crayfish prophenoloxidase leads to increased bacterial growth, lower phagocytosis, lower phenoloxidase activity, lower nodule formation, and higher mortality when infected with this bacterium. In contrast, if RNA interference of pacifastin, an inhibitor of the crayfish prophenoloxidase activation cascade, is performed, it results in lower bacterial growth, increased phagocytosis, increased nodule formation, higher phenoloxidase activity, and delayed mortality. Our data therefore suggest that phenoloxidase is required in crayfish defense against an infection by A. hydrophila, a highly virulent and pathogenic bacterium to crayfish.

Place, publisher, year, edition, pages
2007. Vol. 282, no 46, 33593-33598 p.
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-97411DOI: 10.1074/jbc.M706113200ISI: 000250840200040PubMedID: 17855335OAI: oai:DiVA.org:uu-97411DiVA: diva2:172352
Available from: 2008-08-18 Created: 2008-08-18 Last updated: 2011-01-18Bibliographically approved
In thesis
1. Functional Studies of Some Immune Relevant Genes in a Crustacean
Open this publication in new window or tab >>Functional Studies of Some Immune Relevant Genes in a Crustacean
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The freshwater crayfish, Pacifastacus leniusculus, mounts a strong innate immune response against microbes such as viruses and bacteria. In this thesis, a novel RNA interference (RNAi) method mediated with histone H2A was developed and applied in crayfish hematopoietic tissue cell cultures for gene functional studies. Further, the interactions between host (crayfish) and pathogens (white spot syndrome virus and Aeromonas hydrophila, respectively) were studied using RNAi technology in live animals. An antilipopolysaccharide factor isolated from viral challenged crayfish by suppression subtractive hybridization was shown to interfere with the propagation of white spot syndrome virus both in vivo and in vitro in crayfish, suggesting an important role of this factor in antiviral defense. Besides, RNAi of phenoloxidase, a critical immune effector involved in melanization, revealed that phenoloxidase activity is necessary for crayfish immune defense against a highly pathogenic bacterial infection in crayfish. In addition, RNAi was also employed to study a marker protein gene involved in hemocyte maturation in crayfish. Taken together, these studies may provide more insights into the immune responses against pathogen invasion as well as hemocyte ontogenesis in crustaceans.

Place, publisher, year, edition, pages
Uppsala: Universitetsbiblioteket, 2008. 61 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 453
Keyword
anti-LPS factor, crayfish, hemocyte, innate immunity, melanization, phenoloxidase, RNA interference, WSSV
National Category
Biological Sciences
Identifiers
urn:nbn:se:uu:diva-9194 (URN)978-91-554-7252-8 (ISBN)
Public defence
2008-09-12, Lindahlsalen, EBC, Norbyvägen 18A, Uppsala, 10:00 (English)
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Available from: 2008-08-18 Created: 2008-08-18 Last updated: 2009-04-05Bibliographically approved

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Jiravanichpaisal, PikulCerenius, LageSöderhäll, IreneSöderhäll, Kenneth

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