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Imaging of aromatase distribution in rat and rhesus monkey brains with [C-11]vorozole
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
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2006 (English)In: Nuclear Medicine and Biology, ISSN 0969-8051, E-ISSN 1872-9614, Vol. 33, no 5, 599-605 p.Article in journal (Refereed) Published
Abstract [en]

Aromatase is an enzyme that converts androgens to estrogens and may play a role in mood and mental status. The aim of this study was to demonstrate that brain aromatase distribution could be evaluated with a novel positron emission tomography (PET) tracer [C-11]vorozole. Vorozole is a nonsteroidal aromatase inhibitor that reversibly binds to the heme domain of aromatase. In vitro experiments in rat brain, using frozen section autoradiography, illustrated specific binding in the medial amygdala (MA), the bed nucleus of stria terminalis (BST) and the preoptic area (POA) of male rat brain. Specific binding in female rat brain was found in the MA and the BST; however, the signals were lower than those of males. The K-d of [C-11]vorozole binding to aromatase in MA was determined to be 0.60 +/- 0.06 nM by Scatchard plot analysis using homogenates. An in vivo PET study in female rhesus monkey brain demonstrated the uptake of [C-11]vorozole in the amygdala, where the uptake was blocked by the presence of excess amounts of unlabeled vorozole. Thus, this tracer has a high affinity for brain aromatase and could have a potential for in vivo aromatase imaging. This technique might enable the investigation of human brain aromatase in healthy and diseased persons.

Place, publisher, year, edition, pages
2006. Vol. 33, no 5, 599-605 p.
Keyword [en]
positron emission tomography, aromatase, vorozole, Amygdala, hypothalamus, sex steroids
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-97475DOI: 10.1016/j.nucmedbio.2006.03.009ISI: 000239530200003PubMedID: 16843834OAI: oai:DiVA.org:uu-97475DiVA: diva2:172442
Available from: 2008-09-08 Created: 2008-09-08 Last updated: 2017-12-14Bibliographically approved
In thesis
1. Imaging brain aromatase by using PET: A way to study anabolic steroid abuse
Open this publication in new window or tab >>Imaging brain aromatase by using PET: A way to study anabolic steroid abuse
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Aromatase is an enzyme that facilitates the conversion of androgens to estrogens and may play a role in mood and mental status. The main theme of this thesis is the imaging of brain aromatase by use of the PET technique. The PET tracer for aromatase, 11C-labeled vorozole (VOZ) was developed and evaluated by with in vitro and in vivo methods. In vitro experiments using rat brain showed that VOZ was distributed in the medial amygdala, bed nucleus of the stria terminalis and medial preoptic area, regions of the brain known to be rich in aromatase and the KD value was determined to be 0.60 nM. The in vivo PET study in rhesus monkey brain revealed that VOZ penetrated the blood-brain barrier and accumulated in the amygdala and hypothalamus. Taken together, VOZ is a good PET tracer for in vivo aromatase imaging with high affinity and high sensitivity.

This technique was applied to an investigation of brain aromatase under the physiological conditions simulating anabolic-androgenic steroid abuse. A significant increase in VOZ binding by anabolic-androgenic steroids was observed in the bed nucleus of stria terminalis and medial preoptic area in the rat brain. In contrast, no significant change in binding was observed in the medial amygdala. These results indicate that the manner of regulation of aromatase expression might be different in the bed nucleus of stria terminalis and medial preoptic area compared with that in the medial amygdala. The aromatase expression was suggested to be regulated through androgen receptors, as indicated in a study with flutamide treatment. The increased aromatase expression was seen in neurons. The PET study with anabolic steroid-treated rhesus monkeys also showed increased VOZ binding in the hypothalamus but not in the amygdala. The alteration of density of aromatase binding in the hypothalamic area could explain some psychological features of anabolic-androgenic steroid abusers.

Novel PET tracers for aromatase were developed and examined. The two newly synthesized 18F-labeled vorozole analogs, [18F]FVOZ and [18F]FVOO, displayed different characteristics. Both tracers showed similar binding pattern as VOZ; however, [18F]FVOO was metabolized very quickly, meaning that this tracer is not suitable as a PET tracer. On the other hand, [18F]FVOZ can be an appropriate PET tracer.

The role of aromatase in the human brain has not been clarified yet. To approach this problem by in vivo methods, we have just started PET studies to explore aromatase expression in humans.

Place, publisher, year, edition, pages
Uppsala: Universitetsbiblioteket, 2008. 63 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 370
Keyword
aromatase, brain, molecular imaging, PET, [11C]Vorozole, amygdala, hypothalamus, anabolic-androgenic steroids, abuse, [18F]Vorozole analogs
National Category
Neurosciences
Identifiers
urn:nbn:se:uu:diva-9234 (URN)978-91-554-7266-5 (ISBN)
Public defence
2008-10-04, B42, A4, BMC, Uppsala, 09:15
Opponent
Supervisors
Available from: 2008-09-08 Created: 2008-09-08Bibliographically approved

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