Injectable cell-free template for bone-tissue formation
2009 (English)In: Journal of Biomedical Materials Research-Part A, ISSN 1549-3296, Vol. 91A, no 4, 1111-1118 p.Article in journal (Refereed) Published
Here we present a novel injectable hydrogel which forms a template for de novo formation of bone tissue. Hydrogel formation takes place in situ in less than 1 min by the cross-linking of multifunctional hyaluronic acid and polyvinyl alcohol derivatives. Endogenous cells are recruited in vivo by incorporating bone morphogenetic protein-2 (BMP-2), a powerful promoter for osteogenic differentiation. The hydrogel was evaluated in vitro by performing a cell viability test and a release study and in vivo by a rat ectopic model. Examination by X-ray, microcomputed tomography, and histology revealed a significant bone formation at the target site for gels containing BMP-2, and a complete degradation was observed for gels without BMP-2 four weeks after injection. There were no signs of inflammation or foreign body response in either group and we believe that this system has the potential as an off-the-shelf injectable to be used where bone tissue is needed.
Place, publisher, year, edition, pages
Wiley Periodicals, Inc , 2009. Vol. 91A, no 4, 1111-1118 p.
hydrogel, hyaluronan, injectable, bone tissue engineering, bone morphogenetic protein, polyvinyl alcohol
Research subject Inorganic Chemistry
IdentifiersURN: urn:nbn:se:uu:diva-97730DOI: 10.1002/jbm.a.32289ISI: 000272196900017OAI: oai:DiVA.org:uu-97730DiVA: diva2:172780