Isolation and properties of the CYP2D25 promoter: Transcriptional regulation by vitamin D3 metabolites
2006 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 345, no 2, 568-572 p.Article in journal (Refereed) Published
Previous studies have suggested that hepatic production of 25-hydroxyvitamin D3 may be suppressed by 1α,25-dihydroxyvitamin D3. However, the molecular details of these observations have not been clarified. In the current study, the 5´-flanking DNA sequence of CYP2D25, a porcine microsomal vitamin D 25-hydroxylase, was isolated and analyzed. The CYP2D25 promoter contains a putative vitamin D response element (VDRE). The promoter activity was markedly suppressed by 1α,25-dihydroxyvitamin D3 and 25-hydroxyvitamin D3 in presence of vitamin D receptor (VDR). The data suggest that VDR-mediated inhibition of 25-hydroxylase(s) by vitamin D3 metabolites at the transcriptional level may play an important role in the regulation of 25-hydroxyvitamin D3 production in liver and other tissues.
Place, publisher, year, edition, pages
2006. Vol. 345, no 2, 568-572 p.
Vitamin D3, 25-Hydroxylase, CYP2D25, Promoter, Transcriptional regulation
IdentifiersURN: urn:nbn:se:uu:diva-97879DOI: 10.1016/j.bbrc.2006.04.116PubMedID: 16690021OAI: oai:DiVA.org:uu-97879DiVA: diva2:172974