Capillary electrophoresis off-line matrix-assisted laser desorption/ionisation mass spectrometry of intact and digested proteins using cationic-coated capillaries
2004 (English)In: Rapid Communications in Mass Spectrometry, ISSN 0951-4198, E-ISSN 1097-0231, Vol. 18, no 23, 2946-2952 p.Article in journal (Refereed) Published
Capillary electrophoresis (CE) was coupled off-line with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS) for the analysis of proteins and peptides. CE fractions were collected directly on a matrix-coated MALDI target, using a sheath-flow interface. Protein adsorption during CE separations was prevented by coating the capillaries with the physically adsorbed, cationic polymer PolyE-323. The CE/MALDI-MS system was used for the analysis of model proteins and peptides at physiological pH as well as analysis of proteins in tear fluid. Moreover, tryptic on-target digestion of the collected protein fractions, with subsequent MALDI-MS and MS/MS peptide analysis, was demonstrated.
Place, publisher, year, edition, pages
2004. Vol. 18, no 23, 2946-2952 p.
Chemical Sciences Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-98066DOI: 10.1002/rcm.1712PubMedID: 15529414OAI: oai:DiVA.org:uu-98066DiVA: diva2:173236