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Genome-wide identification of quantitative trait loci in a cross between Hampshire and Landrace I: carcass traits
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2006 (English)In: Animal Genetics, ISSN 0268-9146, E-ISSN 1365-2052, Vol. 37, no 2, 156-162 p.Article in journal (Refereed) Published
Abstract [en]

 

We report the identification of quantitative trait loci (QTL) affecting carcass composition, carcass length, fat deposition and lean meat content using a genome scan across 462 animals from a combined intercross and backcross between Hampshire and Landrace pigs. Data were analysed using multiple linear regression fitting additive and dominance effects. This model was compared with a model including a parent-of-origin effect to spot evidence of imprinting. Several precisely defined muscle phenotypes were measured in order to dissect body composition in more detail. Three significant QTL were detected in the study at the 1% genome-wide level, and twelve significant QTL were detected at the 5% genome-wide level. These QTL comprise loci affecting fat deposition and lean meat content on SSC1, 4, 9, 10, 13 and 16, a locus on SSC2 affecting the ratio between weight of meat and bone in back and weight of meat and bone in ham and two loci affecting carcass length on SSC12 and 17. The well-defined phenotypes in this study enabled us to detect QTL for sizes of individual muscles and to obtain information of relevance for the description of the complexity underlying other carcass traits.

Place, publisher, year, edition, pages
2006. Vol. 37, no 2, 156-162 p.
Keyword [en]
carcass traits, genome scan, Hampshire, Landrace, quantitative trait loci
National Category
Medical and Health Sciences
Research subject
Genetics
Identifiers
URN: urn:nbn:se:uu:diva-98075DOI: 10.1111/j.1365-2052.2005.01405.xPubMedID: 16573530OAI: oai:DiVA.org:uu-98075DiVA: diva2:173247
Available from: 2009-02-20 Created: 2009-02-10 Last updated: 2017-12-13Bibliographically approved
In thesis
1. QTL Analysis in the Pig: From the Identification of Quantitative Trait Loci to the Understanding of Molecular Mechanisms
Open this publication in new window or tab >>QTL Analysis in the Pig: From the Identification of Quantitative Trait Loci to the Understanding of Molecular Mechanisms
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Domestic pigs have become very different form the wild ancestors they originate from. Selection for muscle growth and meat quality has made the pig a good model for genetic studies of muscle development.

The first part of this thesis presents a genome-wide scan for quantitative trait loci (QTL) in a cross between Landrace and Hampshire pigs. Traits such as body composition, fat deposition, body length, meat quality and weight measurements of individual muscles were investigated. In total we identified 15 different QTLs that reached genome-wide significance. The three most significant QTLs were for carcass length on chromosome 17 and two overlapping QTLs on chromosome 1 for body composition and weight of M. biceps femoris, respectively. A strong candidate gene for the body composition QTL is melanocortin 4 receptor (MC4R). We also identified several QTLs for sizes of different muscles, fat deposition and meat quality traits.

In a previous study using a cross between the domestic Large White and wild boar, the mutation underlying a major QTL for muscle growth and fat deposition was identified as a single nucleotide substitution (QTN) in intron 3 of the IGF2 gene. The QTN disrupts the binding of a repressor affecting IGF2 mRNA expression. In the second part of this thesis, the identification of the repressor is presented. The repressor, named ZBED6, is a previously unknown mammalian member of the BED-domain protein family. We could show that Zbed6 specifically binds the wild-type but not the mutated sequence surrounding the QTN. Further studies of silenced Zbed6 in the mouse myoblast cell line C2C12 showed that it represses transcription in a luciferase reporter assay and affects Igf2 mRNA transcription and proliferation. ZBED6 shows very high sequence conservation and has a broad tissue distribution of expression suggesting that ZBED6 also has important biological function outside the muscle cell.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2009. 55 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 420
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-9556 (URN)978-91-554-7412-6 (ISBN)
Public defence
2009-03-13, B41, BMC, Husargatan 3, Uppsala, 13:15 (English)
Opponent
Supervisors
Available from: 2009-02-20 Created: 2009-02-10 Last updated: 2012-11-26Bibliographically approved

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Andersson, Leif

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