This work was about Raman spectroscopy and refractive index and how they could be used tostudy healthy blood. The purpose of the paper was to study how Raman spectroscopy andrefractive index can be used to study blood. Subsequently, this was done through both aqualitative and quantitative study. The qualitative part was about gathering theory andintegrating it with the results. The quantitative part was conducted through empirics. Hence,Raman spectroscopy examined blood from a rabbit. Moreover, two scaffolds were built to studyhow the refractive index was affected by the blood. The result showed that Raman spectroscopyand refractive index both can be used to study blood in the present. Raman spectroscopy canexamine blood through intensity and wavenumber. Consequently, the refractive index shows thatthe larger the area of the blood sample, the smaller the refractive index.