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Lectin affinity chromatography as a tool to differentiate endogenous and recombinant erythropoietins
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology.
2008 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1212, no 1-2, 82-88 p.Article in journal (Refereed) Published
Abstract [en]

This work exploits the combination of the lectin affinity chromatography (LAC) with an ultra-sensitive immunochromatographic assay to differentiate several types of erythropoietin (EPO). The chromatographic behaviours of different commercial types of recombinant human EPO (rhEPO), EPO analogues (Aranesp) and urine human EPO (uhEPO) from healthy individuals on eight lectin-Sepharose columns, have been worked out. Results show that when using wheat germ agglutinin (WGA)-Sepharose columns, a careful desorption regime starting with very low concentration (2mM) of the competitive sugar N-acetylglucosamine (GlcNAc) makes it possible to efficiently distinguish endogenous EPO from recombinant EPO and EPO analogues.

Place, publisher, year, edition, pages
2008. Vol. 1212, no 1-2, 82-88 p.
Keyword [en]
Erythropoietin, Recombinant human EPO, Lectin affinity, Wheat germ agglutinin, Lateral flow test
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-98499DOI: 10.1016/j.chroma.2008.10.036ISI: 000261272800012PubMedID: 18976769OAI: oai:DiVA.org:uu-98499DiVA: diva2:174712
Available from: 2009-02-24 Created: 2009-02-24 Last updated: 2017-12-13Bibliographically approved

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Lönnberg, Maria

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