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Effects of a strongly adsorbed additive on process performance in chiral preparative chromatography
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry.
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2008 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1212, no 1-2, 89-97 p.Article in journal (Refereed) Published
Abstract [en]

The shapes of elution profiles are often significantly influenced by the presence of strongly adsorbed additives in the mobile phase. This aspect needs to be considered in quantitative optimization of preparative chromatography. The theoretical study carried out here is based on available thermodynamic information for the enantiomers of three beta-blockers, alprenolol, propranolol, and atenolol, on a teicoplanin chiral stationary phase (Chirobiotic T) using methanol/acetonitrile as the mobile phase and acetic acid/triethylamine as the additive. The properties of this strong additive made it possible to tune the binary elution profiles in any combination of the following apparent band shapes: anti-Langmuir/anti-Langmuir, anti-Langmuir/Langmuir and Langmuir/Langmuir. Optimization of the productivity and yield, when performing repetitive batch injections, was investigated using the equilibrium dispersive model. We show that it is important to consider the invisible additive perturbation peak when defining the cycle time and therefore a model-based optimization needs to take this into account. Furthermore, both productivity and yield could be improved for the two unusual shape combinations in comparison to the traditional Langmuir/Langmuir case.

Place, publisher, year, edition, pages
2008. Vol. 1212, no 1-2, 89-97 p.
Keyword [en]
Band shape tuning, Unusual band shapes, Adsorption data, Additive, Cycle time, Numerical optimization, Productivity, Yield
National Category
Chemical Sciences
URN: urn:nbn:se:uu:diva-98534DOI: 10.1016/j.chroma.2008.10.040ISI: 000261272800013PubMedID: 18962651OAI: oai:DiVA.org:uu-98534DiVA: diva2:174852
Available from: 2009-02-25 Created: 2009-02-25 Last updated: 2011-03-17Bibliographically approved

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