Sepsis remains a significant global health burden, characterized by a substantial mortality rate. The current diagnostic tools employed to identify and commence appropriate medication, such as antimicrobial susceptibility testing (AST), are burdensome and time-consuming in laboratory settings. However, Q-linea AB has recently introduced an innovative diagnostic instrument known as ASTar, which aims to deliver minimal inhibitory concentration (MIC) values in a fully automated manner within a mere six hours, in contrast to the two-day turnaround time associated with conventional methodologies. By providing a swift and streamlined approach, ASTar has the potential to revolutionize sepsis diagnosis, enabling prompt initiation of effective treatment. The project aims to ensure the quality, safety, and sustainability of the manufacturing process. Key considerations include understanding the interrelationships between formulation, stability, identifying validated cleaning procedures to enhance the manufacturing process more sustainable, safe and efficient. Different experiments were conducted during the project with aim to evaluate establish an effective washing method to remove antibiotic residues from glassware, determine the stability of solvents used in laboratory settings, and validate the cleaning procedure of dispensing machine. The results in washing experiments with glassware indicated that increasing the number of washes effectively reduced the concentration of antibiotics and inhibited microbial growth. The inclusion of abrasive action, combined with soap or Milli-Q water, proved crucial in removing antibiotic residues. The solvent stability testing revealed that the majority of solvents exhibited good pH stability, indicating their suitability for reuse. However, one solvent showed potential degradation over time indicated by change in pH. The cleaning experiment in the dispenser demonstrated the effectiveness of the washing method in reducing antibiotic concentrations below the detection limit. Overall, this thesis report contributes valuable insights into microbial growth inhibition, solvent stability, and antibiotic residue removal, with the aim of improving laboratory practices, enhancing AST instrument cleaning accuracy, and promoting sustainable production processes in microbiology and antimicrobial research. The thesis contributes to the overall vision of the company to provide sustainable solutions to ensure good environment of AST disc production and handling of antimicrobial drugs.