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Occurrence and evolutionary origins of polyploids in the club moss genus Diphasiastrum (Lycopodiaceae)
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Evolution, Genomics and Systematics.
University of Vienna, Department of Systematic and Evolutionary Botany.
Institute of Botany,Chinese Academy of Sciences .
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Evolution, Genomics and Systematics.
2009 (English)In: Molecular Phylogenetics and Evolution, ISSN 1055-7903, E-ISSN 1095-9513, Vol. 52, no 3, 746-754 p.Article in journal (Refereed) Published
Abstract [en]

Two polyploid taxa are commonly recognized in the genus Diphasiastrum, D. wightianum from Asia and D. zanclophyllum from South Africa and Madagascar. Here we present results from Feulgen DNA image densitometry analyses providing the first evidence for the polyploid origin of D. zanclophyllum. Reported for the first time is also data confirming that D. multispicatum and D. veitchii, representing putative parent lineages for D. wightianum, are diploids. Phylogenetic analyses of nuclear regions RPB2, LEAFY and LAMB4 reveal that putative tetraploid accessions are of allopolyploid origin. Diphasiastrum zanclophyllum shows close relationships to the North American taxon D. digitatum on the maternal side, but the paternal relationship is less clear. Two accessions from Asia, both found to be polyploid, have D. veitchii as maternal parent, whereas the paternal paralogs show relationships to D. multispicatum and D. tristachyum, respectively. None of these parental combinations have previously been hypothesized.

Place, publisher, year, edition, pages
2009. Vol. 52, no 3, 746-754 p.
Keyword [en]
Diphasiastrum, Feulgen DNA image densitometry, Lycopodium, Lycopodiaceae, low-copy nuclear genes, phylogenies, polyploidy
National Category
Biological Sciences
Research subject
Systematic Botany
Identifiers
URN: urn:nbn:se:uu:diva-99577DOI: 10.1016/j.ympev.2009.05.004ISI: 000268265800016OAI: oai:DiVA.org:uu-99577DiVA: diva2:208224
Available from: 2009-03-16 Created: 2009-03-16 Last updated: 2010-07-13Bibliographically approved
In thesis
1. Reticulate Evolution in Diphasiastrum (Lycopodiaceae)
Open this publication in new window or tab >>Reticulate Evolution in Diphasiastrum (Lycopodiaceae)
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In this thesis relationships and the occurrence of reticulate evolutionary events in the club moss genus Diphasiastrum are investigated. Diphasiastrum is initially established as a monophyletic group within Lycopodiaceae using non recombinant chloroplast sequence data. Support is obtained for eight distinct parental lineages in Diphasiastrum, and relationships among the putative parent taxa in the hypothesized hybrid complexes; D. alpinum, D. complanatum, D. digitatum, D. multispicatum, D. sitchense, D. tristachyum and D. veitchii are presented.

Feulgen DNA image densitometry data and sequence data obtained from three nuclear regions, RPB2, LEAFY and LAMB4, were used to infer the origins of three different taxa confirmed to be allopolyploid; D. zanclophyllum from South Africa, D. wightianum from Malaysia and an undescribed taxon from China. The two Asian polyploids have originated from two different hybrid combinations, D. multispicatum x D. veitchii and D. tristachyum x D. veitchii. Diphasiastrum zanclophyllum originates from a cross between D. digitatum and an unidentified diploid taxon.

The occurrence of three homoploid hybrid combinations commonly recognized in Europe, D. alpinum x D. complanatum, D. alpinum x D. tristachyum and D. complanatum x D. tristachyum, are verified using the same three nuclear regions. Two of the three hybrid combinations are also shown to have originated from reciprocal crosses. Admixture analyses performed on an extended, dataset similarly identified predominately F1 hybrids and backcrosses. The observations and common recognition of hybrid species in the included populations are hence most likely due to frequent observations of neohybrids in hybrid zones. Reticulate patterns are, however, prominent in the presented dataset. Hence future studies addressing evolutionary and ecological questions in Diphasiastrum should emphasize the impact of gene flow between parent lineages rather than speciation as the result of hybridization.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2009. 35 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 623
Keyword
admixture analysis, Bayesian clustering, Diphasiastrum, Feulgen DNA image densitometry, homoploid hybridization, Lycopodiaceae, phylogenetic analysis, polyploidy, reticulate evolution
National Category
Biological Systematics
Research subject
Systematic Botany
Identifiers
urn:nbn:se:uu:diva-99584 (URN)978-91-554-7470-6 (ISBN)
Public defence
2009-04-29, Lindahlsalen, Norbyvägen 18, Uppsala University, 10:00 (English)
Opponent
Supervisors
Available from: 2009-04-08 Created: 2009-03-16 Last updated: 2009-09-14Bibliographically approved

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Aagaard, Sunniva M. D.

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