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Chitosan as a carrier for non-viral gene transfer in a cystic-fibrosis cell line
Karolinska Institutet.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Karolinska Institutet.
2008 (English)In: Biotechnology and applied biochemistry, ISSN 0885-4513, E-ISSN 1470-8744, Vol. 51, no Pt 4, 153-7 p.Article in journal (Refereed) Published
Abstract [en]

The gene transfer mediated by chitosan in CFBE41o(-) (a cystic-fibrosis bronchial epithelial cell line) and HEK (a human embryonic kidney cell line) has been evaluated. Polyplexes based on chitosan and PEI (polyethyleneimine) using a luciferase and enhanced green fluorescent protein reporter plasmid showed that the transfection efficacy of polyplexes in the CFBE41o(-) cell line was poor compared with that in HEK cells. In the highly differentiated cystic-fibrosis bronchial epithelial cell line the narrow-size-distributed chitosan shows enhanced transfection at a low pH compared with PEI. The enhanced transfection at lower pH could be a result of damage to the cell surface or changes in the cell-surface charge, leading to better penetration of the cell membrane.

Place, publisher, year, edition, pages
2008. Vol. 51, no Pt 4, 153-7 p.
Keyword [en]
chitosan and polyethyleneimine (PEI) cationic polymer-nucleic acid complexes (polyplexes), cystic-fibrosis bronchial epithelial cell line (CFBE4Io(-)), cystic-fibrosis transmembrane conductance regulator (CFTR), firefly luciferase and enhanced green fluorescent protein reporter plasmid (pNGVL-EGFPluc), human embryonic kidney-293 cell line (HEK-293), non-viral gene transfer
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-100462DOI: 10.1042/BA20070197ISI: 000261487700005PubMedID: 18289127OAI: oai:DiVA.org:uu-100462DiVA: diva2:210310
Available from: 2009-03-31 Created: 2009-03-31 Last updated: 2017-12-13Bibliographically approved

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