RNA binding properties of the R3H domain of poly(A)-specific ribonuclease (PARN)
(English)Manuscript (Other academic)
Poly(A)-specific ribonuclease (PARN) is an oligomeric, processive and cap-interacting 3'-5' exoribonuclease that efficiently degrades eukaryotic mRNA poly(A) tails. PARN harbors, in addition to a well characterized RNA-recognition motif (RRM), another putative RNA-binding motif: the R3H-domain. R3H-domains have previously been found in a diverse range of proteins involved in single stranded nucleic acid binding suggesting that the domain is a nucleic acid interacting motif. However, its RNA binding properties has not been experimentally verified. Here, we show that the R3H domain of PARN binds poly(A) and poly(U). Therefore, our study verifies that the R3H domain binds RNA and suggests that the R3H-domain of PARN could play a role in recognizing poly(A).
mRNA degradation, deadenylation, Poly (A)-specific ribonuclease, poly(A)-specificity, R3H-domain
IdentifiersURN: urn:nbn:se:uu:diva-100499OAI: oai:DiVA.org:uu-100499DiVA: diva2:210396