The effect of sample salt additives on capillary electrophoresis analysis of intact proteins using surface modified capillaries.
2009 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1216, no 17, 3613-3620 p.Article in journal (Refereed) Published
The effect of adding alkali salts to protein samples for capillary electrophoretic (CE) analysis of intact proteins was studied. A high degree of peak stacking, even for large proteins, was found to occur when alkali salts were added to the sample. The addition of salt to the protein sample promotes a strong improvement in the peak efficiency of individual proteins giving up to 2.1 x 10(6) apparent plates/m. The concentration of salt required in the sample to reach optimal peak efficiency show dependency on both the molecular weight and molar concentration of the protein. However, adding salt will, at a sufficiently high concentration, cause a mixture of proteins to co-migrate to one very sharp peak. The observed sample stacking effect was obtained with a number of different surface modified silica capillaries indicating a general phenomenon and not surface coating specific.
Place, publisher, year, edition, pages
2009. Vol. 1216, no 17, 3613-3620 p.
Capillary electrophoresis, alkali salt, intact protein analysis, coated capillary, stacking effect
Research subject Analytical Chemistry
IdentifiersURN: urn:nbn:se:uu:diva-100628DOI: 10.1016/j.chroma.2008.12.037ISI: 000265467200004PubMedID: 19150070OAI: oai:DiVA.org:uu-100628DiVA: diva2:210704