Proteomic Characterization of IgY Preparations Purified with a Water Dilution Method
2008 (English)In: Journal of Agricultural and Food Chemistry, ISSN 0021-8561, E-ISSN 1520-5118, Vol. 56, no 24, 11638-11642 p.Article in journal (Refereed) Published
Antigen-specific chicken IgY antibodies have been used for oral immunotherapy as an alternative orcomplement to antibiotics in several studies. The water dilution (WD) method has several advantagesfor purifying IgY. It is rapid, efficient, suitable for large-scale production, and nothing but water isadded. The water-soluble fraction contains other proteins and lipids besides IgY. The protein contentwas characterized by two-dimensional gel electrophoresis (2DGE) and nanoflow liquid chromatographycoupled offline to matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry(nanoLC-MALDI TOF/TOF MS). Protein analysis was complicated due to the large dynamicconcentration range, but 26 proteins could be identified. The relative protein concentrations in differentbatches were very similar according to protein patterns on 1D gels and protein concentrationdeterminations. Thus, the purification method has a high reproducibility. The concentrations ofcholesterols and triglycerides were low and should not have an effect on the plasma levels of treatedpatients. Purification of IgY for oral use with WD is therefore a recommended method.
Place, publisher, year, edition, pages
2008. Vol. 56, no 24, 11638-11642 p.
egg yolk, water dilution method, two-dimensional gel electrophoresis, nanoLC-MALDI TOF/TOF MS
Research subject Analytical Chemistry
IdentifiersURN: urn:nbn:se:uu:diva-100698DOI: 10.1021/jf802626tISI: 000261802400012PubMedID: 19053374OAI: oai:DiVA.org:uu-100698DiVA: diva2:210819