Proteomic analysis of prostate cancer metastasis: derived prostasomes
2010 (English)In: Anticancer Research, ISSN 0250-7005, Vol. 30, no 2, 285-290 p.Article in journal (Refereed) Published
The secretory epithelial cells of the prostate gland use sophisticated vehicles in the form of prostasomes to relay important information to sperm cells in semen. This prostasome-forming and secretory ability of the epithelial cells is also preserved in poorly differentiated prostate cancer cells. We investigated prostasomes from vertebral metastases of prostate cancer, taken from the operating field at surgery, directly taken care of under protease inhibitory conditions for later 2-dimensional electrophoresis and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) protein characterization. A total of 104 spots were punched out for identification. Twenty five unique protein spots had a MALDI-TOF above 49 and another 5 proteins were determined by MS/MS. The remaining 74 spots were either identical to already determined proteins or had no reliable score. Annexins A1, A3, and A5 as well as dimethylarginine dimethylaminohydrolase 1 were among the identified proteins. The annexins and dimethylarginine dimethylaminohydrolase 1 found in cancer-derived prostasomes can act, among other things, as angiogenic factors and can increase the vascular development in the neighborhood of the tumor. Cancer-derived prostasomes may play an important role in the interaction between tumor cells and their environment.
Place, publisher, year, edition, pages
2010. Vol. 30, no 2, 285-290 p.
Metastasis, prostasomes, exosomes, annexin
Medical and Health Sciences
Research subject Clinical Chemistry
IdentifiersURN: urn:nbn:se:uu:diva-100733ISI: 000275821200002PubMedID: 20332430OAI: oai:DiVA.org:uu-100733DiVA: diva2:210996