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A novel B cell-mediated transport of IgE-immune complexes to the follicle of the spleen.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
2008 (English)In: Journal of Immunology, ISSN 0022-1767, E-ISSN 1550-6606, Vol. 180, no 10, 6604-6610 p.Article in journal (Refereed) Published
Abstract [en]

Ag administered i.v. to mice along with specific IgE or IgG2a induces higher Ab- and CD4(+) T cell responses than Ag administered alone. The IgE effect is completely dependent on the low-affinity receptor for IgE, CD23, whereas the IgG2a effect depends on activating FcgammaRs. In vitro studies suggest that IgE/Ag is presented more efficiently than Ag alone to CD4(+) T cells by CD23(+) B cells and that IgG2a/Ag is presented by FcgammaR(+) dendritic cells (DCs). In this study, we investigate in vivo the early events leading to IgE- and IgG2a-mediated enhancement of immune responses. OVA administered i.v. in PBS in combination with specific IgE binds circulating B cells after 5 min and is found in B cell follicles bound to follicular B cells (CD23(high)) after 30 min. This novel B cell-dependent route of entry is specific for IgE because IgG2a-Ag complexes were trapped in the marginal zone. OVA-specific CD4(+) T cells were found at the T-B border in the T cell zones 12 h after immunization both with IgE/OVA or IgG2a/OVA and proliferated vigorously after 3 days. The findings suggest that IgE- and IgG2a-immune complexes are efficient stimulators of early CD4(+) T cell responses and that Ag bound to IgE has a specific route for transportation into follicles.

Place, publisher, year, edition, pages
2008. Vol. 180, no 10, 6604-6610 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-100857ISI: 000257507100024PubMedID: 18453579OAI: oai:DiVA.org:uu-100857DiVA: diva2:211137
Available from: 2009-04-08 Created: 2009-04-08 Last updated: 2011-06-28Bibliographically approved

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Heyman, Birgitta
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