Monitoring minimal residual disease with flow cytometry, antigen-receptor gene rearrangements and fusion transcript quantification in Philadelphia-positive childhood acute lymphoblastic leukemia
2009 (English)In: Leukemia research: a Forum for Studies on Leukemia and Normal Hemopoiesis, ISSN 0145-2126, E-ISSN 1873-5835, Vol. 33, no 8, 1047-1054 p.Article in journal (Refereed) Published
In this study, we followed minimal residual disease (MRD) in eight children with Philadelphia-positive acute lymphoblastic leukemia (Ph+ ALL) using (i) flow cytometry (FCM), (ii) real-time quantitative PCR of IG/TCR gene rearrangements and (iii) RT-PCR detecting fusion gene transcripts. In six of the eight cases the kinetics of MRD clearance was comparable. One of the two discordant cases could be explained by presence of an alternative fusion transcript. The other discordant case showed high BCR-ABL1 RNA level while the other methods did not detect any MRD. In our limited material quantitative RT-PCR of fusion gene transcripts seemed particularly useful to measure MRD in Ph+ ALL. However, BCR-ABL1 expression may not reflect the percentage of leukemic cells as FCM and IG/TCR rearrangement quantification do, and these methods are thus complementary.
Place, publisher, year, edition, pages
2009. Vol. 33, no 8, 1047-1054 p.
MRD, Philadelphia positive ALL, real-time PCR, flow cytometry, antigen receptor genes, fusion gene transcripts, BCR-ABL1 mutations
Medical and Health Sciences
Research subject Molecular Medicine
IdentifiersURN: urn:nbn:se:uu:diva-101000DOI: 10.1016/j.leukres.2008.11.031ISI: 000266759000009PubMedID: 19157547OAI: oai:DiVA.org:uu-101000DiVA: diva2:211630