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Immunospecific antibody concentration in egg yolk of chickens orally immunised with varying doses of bovine serum albumin and the mucosal adjuvant RhinoVax using different immunisation regimes
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Comparative Medicine.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Comparative Medicine.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Comparative Medicine.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Comparative Medicine.
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2006 (English)In: Scandinavian Journal of Laboratory Animal Science, ISSN 0901-3393, Vol. 33, no 3, 163-169 p.Article in journal (Refereed) Published
Abstract [en]

Antibody harvested from eggs of immunised chickens, IgY, has proven to be a non-invasive alternative to antibodies purified from serum of mammals. Taking the non-invasive concept further, the development of oral immunization techniques combined with IgY harvest from chicken eggs may subsequently eliminate all regulated procedures from polyclonal antibody production. In the present study, we report the effects of varying the temporal administration mode of the antigen (immumogen) comparing dosing on three consecutive days with dosing on five consecutive days, and of incorporating a mucosal adjuvant. Two antigen doses were compared: 30 mg bovine serum albumin (BSA) and 300 mg BSA, with and without the mucosal adjuvant, RhinoVax (R), administered to laying chickens. The egg yolk of chickens dosed with BSA in combination with 20% RhinoVax (R), contained significantly higher concentrations of immunospecific IgY than did egg yolks of chickens fed with BSA without adjuvant. The most efficient dose in the RhinoVax (R)-treated groups was 300 mg BSA regardless of whether the chickens were initially immunised daily for three or five days. A 3-day dosing regime with BSA alone also induced immiumospecific IgY production. This study confirms that RhinoVax (R) is an efficient oral adjuvant. It also demonstrates the efficacy of daily immunizations on three or five consecutive days on immunospecific IgY production. The chickens received oral booster immunizations one and two months after the initial immunization. No real effect could be recorded after the second and third immunization, although the study did provide some evidence of memory based on an optimum IgY concentration recorded after the 2(nd) immunization.

Place, publisher, year, edition, pages
2006. Vol. 33, no 3, 163-169 p.
National Category
Biomedical Laboratory Science/Technology
Research subject
Comparative Medicine
Identifiers
URN: urn:nbn:se:uu:diva-101414ISI: 000241584200004OAI: oai:DiVA.org:uu-101414DiVA: diva2:212975
Available from: 2009-04-26 Created: 2009-04-26 Last updated: 2017-12-13Bibliographically approved
In thesis
1. Non-invasive Antibody Production in the Chicken
Open this publication in new window or tab >>Non-invasive Antibody Production in the Chicken
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The production of antibodies for analytical purposes using invasive procedures on small mammals is common practice in biomedical research. The aim of this study was to develop an efficient method for non-invasive antibody production in the chicken. This thesis presents an alternative method that eliminates the discomfort, pain and distress invoked by traditional immunization procedures on mammals by instead harvesting antibodies (IgY) from the yolk of eggs laid by orally immunized hens.

An efficient oral immunization regime was developed by first trying out a suitable non-aggressive oral adjuvant with Bovine Serum Albumine (BSA) as the model antigen. It was found that the pegylated mono/diglyceride RhinoVax® (Softigen®) at a concentration of 20% (v/v) produced a good humoral antibody response in chickens as well as development of IgY antibodies in the egg yolk. The age of the chicken is important in order to have a proper humoral immune response. We found that chicken older than 22 days produced circulating immunospecific anti BSA-antibodies of of IgG, IgM and IgA class when orally immunized with BSA alone, whereas chickens 15 days old only produced IgM and IgA antibodies.

This is the first report of oral immunizations with a high dose (250–300mg) of BSA in 20% RhinoVax® consisting of 3 or 5 consecutive daily doses resulting in high concentrations of immunospecific IgY antibodies in the yolk. Using this technique of three consecutive daily doses repeated after 7 weeks and after 18 weeks, a booster effect was induced after the third immunization. This is the first demonstration of a clear anamnestic immune response in orally immunized chickens. The results suggest that it may be possible to further increase the concentration of immunospecific IgY antibodies by modifying the immunization regime. It seems plausible to develop a procedure where the immunogen can be fed to the chickens as in an ordinary egg producing farm thus making antibody production not classified as an animal experiment.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2009. 64 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 461
Series
National Category
Biomedical Laboratory Science/Technology
Research subject
försöksdjursvetenskap/comparative medicine
Identifiers
urn:nbn:se:uu:diva-102072 (URN)978-91-554-7543-7 (ISBN)
Public defence
2009-06-12, Lektionssal B7:101, Uppsala Biomedicinska Centrum - BMC, Husargatan 8, Uppsala, 09:15 (English)
Opponent
Supervisors
Available from: 2009-05-19 Created: 2009-04-30 Last updated: 2009-05-19Bibliographically approved

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Mayo, Susan

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