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Enhancement of anamnestic immunospecific antibody response in orally immunised chickens
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Comparative Medicine.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Comparative Medicine.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Comparative Medicine.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Comparative Medicine.
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2009 (English)In: Journal of Immunological Methods, ISSN 0022-1759, Vol. 342, no 1-2, 58-63 p.Article in journal (Refereed) Published
Abstract [en]

Production of immunospecific egg yolk antibodies (IgY antibodies) in egg laying hens through oral immunization is an attractive alternative to conventional antibody production in mammals for economic reasons as well as for animal welfare reasons. oral immunization results in a systemic humoral response, but oral booster immunizations lack efficiency. The aim of the present study was to develop immunization schemes in which the concentration of immunospecific IgY would increase following oral booster immunizations. Two groups of egg laying hens (5 in each group) were immunized orally (each immunization event consisted of dosing on three consecutive days) with Bovine Serum Albumin (BSA) in combination with RhinoVax (R) (RV) using different immunization schemes. A 3rd group served as a reference and received BSA emulsified in Freund's Incomplete Adjuvant (FIA) by subcutaneous injection three times and one oral dose with BSA+RV. The eggs of the chickens in this group had a significantly higher immunospecific anti BSA IgY-concentration than did any of the eggs from the orally immunized chickens. One of the immunization regimes (immunizations in weeks 1, 7 and 18) clearly included a booster effect of the immunization in week 18, demonstrating the presence of memory cells following the two initial oral immunizations. Considering that oral immunization results in approximately ten times lower concentrations of immunospecific antibodies in the egg yolk, compared to traditional subcutaneous immunization schemes, the oral immunization routines have to be further refined to compete with parenteral immunization protocols.

Place, publisher, year, edition, pages
2009. Vol. 342, no 1-2, 58-63 p.
Keyword [en]
IgY antibodies, Oral immunization, Immunological memory, Animal welfare
National Category
Medical and Health Sciences
Research subject
Comparative Medicine
Identifiers
URN: urn:nbn:se:uu:diva-101415DOI: 10.1016/j.jim.2008.11.017ISI: 000264685300006OAI: oai:DiVA.org:uu-101415DiVA: diva2:212976
Available from: 2009-04-26 Created: 2009-04-26 Last updated: 2010-08-11Bibliographically approved
In thesis
1. Non-invasive Antibody Production in the Chicken
Open this publication in new window or tab >>Non-invasive Antibody Production in the Chicken
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The production of antibodies for analytical purposes using invasive procedures on small mammals is common practice in biomedical research. The aim of this study was to develop an efficient method for non-invasive antibody production in the chicken. This thesis presents an alternative method that eliminates the discomfort, pain and distress invoked by traditional immunization procedures on mammals by instead harvesting antibodies (IgY) from the yolk of eggs laid by orally immunized hens.

An efficient oral immunization regime was developed by first trying out a suitable non-aggressive oral adjuvant with Bovine Serum Albumine (BSA) as the model antigen. It was found that the pegylated mono/diglyceride RhinoVax® (Softigen®) at a concentration of 20% (v/v) produced a good humoral antibody response in chickens as well as development of IgY antibodies in the egg yolk. The age of the chicken is important in order to have a proper humoral immune response. We found that chicken older than 22 days produced circulating immunospecific anti BSA-antibodies of of IgG, IgM and IgA class when orally immunized with BSA alone, whereas chickens 15 days old only produced IgM and IgA antibodies.

This is the first report of oral immunizations with a high dose (250–300mg) of BSA in 20% RhinoVax® consisting of 3 or 5 consecutive daily doses resulting in high concentrations of immunospecific IgY antibodies in the yolk. Using this technique of three consecutive daily doses repeated after 7 weeks and after 18 weeks, a booster effect was induced after the third immunization. This is the first demonstration of a clear anamnestic immune response in orally immunized chickens. The results suggest that it may be possible to further increase the concentration of immunospecific IgY antibodies by modifying the immunization regime. It seems plausible to develop a procedure where the immunogen can be fed to the chickens as in an ordinary egg producing farm thus making antibody production not classified as an animal experiment.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2009. 64 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 461
Series
National Category
Biomedical Laboratory Science/Technology
Research subject
försöksdjursvetenskap/comparative medicine
Identifiers
urn:nbn:se:uu:diva-102072 (URN)978-91-554-7543-7 (ISBN)
Public defence
2009-06-12, Lektionssal B7:101, Uppsala Biomedicinska Centrum - BMC, Husargatan 8, Uppsala, 09:15 (English)
Opponent
Supervisors
Available from: 2009-05-19 Created: 2009-04-30 Last updated: 2009-05-19Bibliographically approved

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