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Systemic immune response of young chickens orally immunized with bovine serum albumin
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Comparative Medicine.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Comparative Medicine.
2003 (English)In: In Vivo, ISSN 0258-851X, Vol. 17, no 3, 261-268 p.Article in journal (Refereed) Published
Abstract [en]

The efficacy of oral immunization, with and without commercial adjuvants, to mount a systemic immune response in young chickens was studied. Bovine serum albumin (BSA) mixed with a pegylated C8/C10 mono/di-glyceride, (Softigen(R)), or Cholera toxin B-subunit (CTB), administered orally by gavage to 15-day-old chickens resulted in circulating immunospecific anti-BSA IgG, IgM and IgA antibodies. Continuous 5-day oral administration of BSA without adjuvant also resulted in immunospecific IgM and 1&A antibodies in the circulation of chickens first immunized at 15 days of age; and immunospecific antibodies of all three classes in chickens first immunized when they were 22 days old. IgG and IgM serum concentrations were more than 4 to 10 times higher, respectively, in CTB- and Softigen-treated chickens as compared to chickens immunized without adjuvants. The IgA response in the orally immunized chickens seemed unaffected by CTB and Softigen. The antibody concentrations in chickens immunized subcutaneously with BSA emulsified in Freund's Incomplete Adjuvant (FIA) were approximately 10 times higher than those of the chickens orally immunized using CTB and Softigen.

Place, publisher, year, edition, pages
2003. Vol. 17, no 3, 261-268 p.
Keyword [en]
oral immunization, cholera toxin B-subunit, pegylated C8/C10 mono/diglyce ride, softigen, IgG, IgA, IgM, bovine serum albumin, chickens, gallus domesticus, Freund's adjuvant
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-102071ISI: 000184551000011PubMedID: 12929578OAI: oai:DiVA.org:uu-102071DiVA: diva2:214025
Available from: 2009-04-30 Created: 2009-04-30 Last updated: 2011-11-02Bibliographically approved
In thesis
1. Non-invasive Antibody Production in the Chicken
Open this publication in new window or tab >>Non-invasive Antibody Production in the Chicken
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The production of antibodies for analytical purposes using invasive procedures on small mammals is common practice in biomedical research. The aim of this study was to develop an efficient method for non-invasive antibody production in the chicken. This thesis presents an alternative method that eliminates the discomfort, pain and distress invoked by traditional immunization procedures on mammals by instead harvesting antibodies (IgY) from the yolk of eggs laid by orally immunized hens.

An efficient oral immunization regime was developed by first trying out a suitable non-aggressive oral adjuvant with Bovine Serum Albumine (BSA) as the model antigen. It was found that the pegylated mono/diglyceride RhinoVax® (Softigen®) at a concentration of 20% (v/v) produced a good humoral antibody response in chickens as well as development of IgY antibodies in the egg yolk. The age of the chicken is important in order to have a proper humoral immune response. We found that chicken older than 22 days produced circulating immunospecific anti BSA-antibodies of of IgG, IgM and IgA class when orally immunized with BSA alone, whereas chickens 15 days old only produced IgM and IgA antibodies.

This is the first report of oral immunizations with a high dose (250–300mg) of BSA in 20% RhinoVax® consisting of 3 or 5 consecutive daily doses resulting in high concentrations of immunospecific IgY antibodies in the yolk. Using this technique of three consecutive daily doses repeated after 7 weeks and after 18 weeks, a booster effect was induced after the third immunization. This is the first demonstration of a clear anamnestic immune response in orally immunized chickens. The results suggest that it may be possible to further increase the concentration of immunospecific IgY antibodies by modifying the immunization regime. It seems plausible to develop a procedure where the immunogen can be fed to the chickens as in an ordinary egg producing farm thus making antibody production not classified as an animal experiment.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2009. 64 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 461
Series
National Category
Biomedical Laboratory Science/Technology
Research subject
försöksdjursvetenskap/comparative medicine
Identifiers
urn:nbn:se:uu:diva-102072 (URN)978-91-554-7543-7 (ISBN)
Public defence
2009-06-12, Lektionssal B7:101, Uppsala Biomedicinska Centrum - BMC, Husargatan 8, Uppsala, 09:15 (English)
Opponent
Supervisors
Available from: 2009-05-19 Created: 2009-04-30 Last updated: 2009-05-19Bibliographically approved

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Mayo, SusanPersdotter Hedlund, Gabriella

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