Multiplex Detection of DNA Sequences Using the Volume-Amplified Magnetic Nanobead Detection Assay
2009 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 81, no 9, 3398-3406 p.Article in journal (Refereed) Published
The possibility for conducting multiplex detection of DNA-sequences using the volume-amplified magnetic nanobead detection assay[Stromberg, M.; Goransson, J.; Gunnarsson, K; Nilsson, M.; Svedlindh, P.; Stromme, M. Nano Lett. 2008, 8, 816-821] was investigated. In this methodology, a batch consisting of a mixture of several sizes of probe-tagged magnetic beads was used for detection of several types of targets in the same compartment Furthermore, a nonlinear least-squares deconvolution procedure of the composite imaginary part of complex magnetization vs frequency spectra based on the Cole-Cole model was applied to analyze the data. The results of a quantitative biplex analysis experiment were compared with the corresponding separate single-target assays. Finally, triplex analysis was briefly demonstrated qualitatively. Biplex and triplex detection were found to perform well qualitatively. Biplex detection was found to enable a rough target quantification. Multiplex detection may become a complement to performing multiple separate single-target assays for, e.g., parallel detection of multiple infectious pathogens. Multiplex detection also permits robust relative quantification and inclusion of an internal control to improve quantification accuracy.
Place, publisher, year, edition, pages
2009. Vol. 81, no 9, 3398-3406 p.
Engineering and Technology
Research subject Engineering Science with specialization in Nanotechnology and Functional Materials
IdentifiersURN: urn:nbn:se:uu:diva-102082DOI: 10.1021/ac900561rISI: 000265632400029PubMedID: 19334737OAI: oai:DiVA.org:uu-102082DiVA: diva2:214141