Effect of subcutaneous injection and oral voluntary ingestion of buprenorphine on post-operative serum corticosterone levels in male rats
2008 (English)In: European Surgical Research, ISSN 0014-312X, E-ISSN 1421-9921, Vol. 41, no 3, 272-278 p.Article in journal (Refereed) Published
BACKGROUND: Adequate peri-operative analgesia may reduce post-operative stress response and improve recovery in laboratory animals. We have established a method involving repeated automated blood sampling, allowing quantification of serum corticosterone levels in rats for stress assessment without stress-inducing handling or restraint. In the present study, the effects of the commonly used route of buprenorphine administration (0.05 mg/kg injected subcutaneously) were compared with oral administration (0.4 mg/kg mixed with Nutella and orally administered by voluntary ingestion) in male Sprague-Dawley rats. METHODS: A catheter was placed in the jugular vein and attached to an Accusampler for automated blood sampling. During 96 h after surgery, blood was collected at specified time points. Pre- and post-operative body weights and water consumption were registered. RESULTS: Buprenorphine significantly suppressed levels of circulating corticosterone after the oral but not after the subcutaneous treatment. Both buprenorphine treatments had a positive impact on maintenance of body weight and water consumption, compared to the control group that received no buprenorphine. CONCLUSION: The present investigation suggests that oral voluntary ingestion ad libitum is an efficacious, convenient and non-invasive way of administering peri-operative buprenorphine to rats, as judged by corticosteroid response and effects on body weight and water consumption.
Place, publisher, year, edition, pages
2008. Vol. 41, no 3, 272-278 p.
Surgical stress, Analgesia, Rats
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-102299DOI: 10.1159/000142372ISI: 000260241300004PubMedID: 18596376OAI: oai:DiVA.org:uu-102299DiVA: diva2:214604