Closed head injury in a mouse model results in molecular changes indicating inflammatory responses
2009 (English)In: Journal of Neurotrauma, ISSN 0897-7151, E-ISSN 1557-9042, Vol. 26, no 8, 1307-1314 p.Article in journal (Refereed) Published
Cerebral gene expression changes in response to traumatic brain injury will provide useful information in the search for future trauma treatment. In order to characterize the outcome of mild brain injury, we studied C57BL/6J mice in a weight-drop (30 g), closed head injury model. At various times post-injury, mRNA was isolated from neocortex and hippocampus and transcriptional alterations were studied using quantitative reverse transcriptase PCR and gene array analysis. At three days post-injury, the results showed unilateral injury responses, both in neocortex and hippocampus, with the main effect seen on the side of the skull hit by the dropping weight. Upregulated transcripts encoded products characterizing reactive astrocytes, phagocytes, microglia and immune-reactive cells. Markers for oligodendrocytes and T-cells were not altered. Notably, strong differences in the responses among individual mice were seen, e.g. for the Gfap transcript expressed by reactive astrocytes and the chemokine Ccl3 transcript expressed by activated microglial cells. In conclusion, mild TBI chiefly activates transcripts leading to tissue remodeling, inflammatory processes and chemokine signalling, as in focal brain injury, suggesting putative targets for drug development.
Place, publisher, year, edition, pages
2009. Vol. 26, no 8, 1307-1314 p.
closed head injury, GeneChip array, inflammation, mild trauma, quantitative RT-PCR, weight drop impact
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-102575DOI: 10.1089/neu.2008-0676ISI: 000268629900013PubMedID: 19317611OAI: oai:DiVA.org:uu-102575DiVA: diva2:216409