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Absence of tissue-bound semicarbazide-sensitive amine oxidase activity in carp tissues
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2007 (English)In: Life Sciences, ISSN 0024-3205, E-ISSN 1879-0631, Vol. 80, no 12, 1094-1099 p.Article in journal (Refereed) Published
Abstract [en]

We have previously reported that carp (Cyprinus carpio) tissue mitochondria contain a novel form of monoamine oxidase (MAO), which belongs neither to MAO-A nor to MAO-B of the mammalian enzyme. This conclusion results from the findings that the carp MAO was equally sensitive to a selective MAO-A inhibitor clorgyline and to the MAO-B selective inhibitor l-deprenyl, when tyramine, a substrate for both forms, serotonin or beta-phenylethylamine, a substrate for either A or B-form of mammalian MAO, was used. In the present study, we tried to detect another amine oxidase, termed tissue-bound semicarbazide-sensitive amine oxidase (SSAO), activity in carp tissues. As definition of SSAO was used, such as insensitivity to inhibition of the kynuramine oxidizing activity by an MAO inhibitor pargyline and high sensitivity to the SSAO inhibitor semicarbazide. The results indicated that the oxidizing activity was selectively and almost completely inhibited by 0.1 mM pargyline alone or a combination of 0.1 mM pargyline plus 0.1 mM semicarbazide, but not by 0.1 mM semicarbazide alone. We also tried to detect any SSAO activity by changing experimental conditions, such as lower incubation temperature, higher enzyme protein concentration, a lower substrate concentration and different pH's in the reaction, as the enzyme source. However, still no SSAO activity could be detected in the tissues. These results conclusively indicate that carp tissues so far examined do not contain SSAO activity.

Place, publisher, year, edition, pages
2007. Vol. 80, no 12, 1094-1099 p.
Keyword [en]
Semicarbazide-sensitive amine oxidase (SSAO), Carp (Cyprinus carpio), Monoamine oxidase (MAO), Tissue-bound form, Pargyline, Semicarbazide
National Category
Pharmaceutical Sciences Medical and Health Sciences Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-102693DOI: 10.1016/j.lfs.2006.11.050ISI: 000244766000004PubMedID: 17215008OAI: oai:DiVA.org:uu-102693DiVA: diva2:216666
Available from: 2009-05-11 Created: 2009-05-11 Last updated: 2011-02-11Bibliographically approved

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