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Role of TAB1 in nitric oxide-induced p38 activation in insulin-producing cells
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
2007 (English)In: International Journal of Biological Sciences, ISSN 1449-2288, Vol. 3, no 2, 71-76 p.Article in journal (Refereed) Published
Abstract [en]

The aim of present study was to elucidate the role of TAB1 in nitric oxide-induced activation of p38 MAPK. For this purpose we over-expressed TAB1 in insulin-producing beta-TC6 cells. We observed in cells transiently over-expressing TAB1 that p38 activation was enhanced in response to DETA/NONOate. A lowering of TAB1 levels, using the siRNA technique, resulted in the opposite effect. The DETA/NONOate-induced cell death rate was increased in cells transiently overexpressing TAB1. In stable beta-TC6 cell clones with very high TAB1 levels p38 phosphorylation was enhanced also at basal conditions. DETA/NONOate increased also the phosphorylation of JNK and ERK in beta-TC6 cells, but these events were not affected by TAB1. Interestingly, the inhibitory effect of SB203580 on p38 phosphorylation was paralleled by a stimulatory effect on JNK phosphorylation and an inhibitory effect on ERK phosphorylation. In summary, we propose that TAB1 promotes nitric oxide-induced p38 autophosphorylation. In addition, nitric oxide-induced p38 activation seems to promote JNK inhibition and ERK activation, but this effect appears to not require TAB1. A better understanding of how the TAB1/p38 pathway promotes beta-cell death in response to nitric oxide might help in the development of novel pharmacological approaches in the treatment of diabetes.

Place, publisher, year, edition, pages
2007. Vol. 3, no 2, 71-76 p.
Keyword [en]
apoptosis, nitric oxide, insulin producing cell, TAB1, p38 MAPK
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-100523ISI: 000246980600001PubMedID: 17205106OAI: oai:DiVA.org:uu-100523DiVA: diva2:218268
Available from: 2009-05-19 Created: 2009-04-01 Last updated: 2011-01-28Bibliographically approved

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